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    High‑Performance Liquid Chromatography‑Fingerprint Analyses, In vitro Cytotoxicity, Antimicrobial and Antioxidant Activities of the Extracts of Two Cestrum Species Growing in Egypt

    Sami M. Nasr1★, Mosad A. Ghareeb2, Mona A. Mohamed2, Nehal M. Elwan3, Abd El-Wanes Anter Abdel-Aziz2, Mohamed S. Abdel-Aziz4★ Corresponding author

    1. 1Biochemistry and Molecular Biology Department, Theodor Bilharz Research Institute, EGYPT.
    2. 2Medicinal Chemistry Department, Theodor Bilharz Research Institute, Kornaish El-Nile, 12411 Warrak El-Hadar, Imbaba (P.O. 30), EGYPT.
    3. 3Chemistry Department, Faculty of Science, Cairo University, Giza, EGYPT.
    4. 4Microbial Chemistry Department, Genetic Engineering and Biotechnology Division, National Research Centre, El-Bohouth Street 33, Dokki-Giza, EGYPT.

    CORRESPONDENCE

    Sami M. Nasr

    Biochemistry and Molecular Biology Department, Theodor Bilharz Research Institute, EGYPT.

    samitbri@gmail.com

    Volume 10, Issue 2 · pp. 173–180 · PUBLISHED · DOI: 10.4103/pr.pr_145_17

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Background: Cancer diseases and microbial resistance are serious health disorders associated with oxidative stress and infectious diseases. Their risks can be reducing via using polyphenols-rich plants. Methodology:Different solvent extracts from two Cestrum species (Cestrum nocturnum and Cestrum elegans) were evaluated for their biological and chemical activities. Also, the chemical profiles of the most promising extracts were investigated via high‑performance liquid chromatography (HPLC)‑fingerprint analyses. Results:The tested extracts showed weak to moderate cytotoxicity against Vero cell line with IC50 values ranged from 133.67 μg/ ml to 57.634 μg/ml. The only noncytotoxic extractive fraction was the dichloromethane extract of C. elegans leaves with an IC50 value of 204.732 μg/ml, while the most toxic extract was the ethyl acetate extract of C. elegans flowers with an IC50 value of 19.22 μg/ml. The antimicrobial activity results revealed that the n‑BuOH extract of C. nocturnumwas the most active against four tested microbial strains with inhibition zones (10–13 mm). Also, the water and n‑BuOH extracts of C. elegansleave exhibited moderate activities with inhibition zones (7–9 mm), while for C. elegans flowers both of water and methanol extracts showed strong activities (9–14 mm). In the 2,2’‑diphenyl‑1‑picrylhydrazyl assay, the most active fraction was EtOAc with IC50 values of 100.52 μg/ml and 64.40 μg/ml for C. elegans leaves and flowers respectively, while for C. nocturnum the most active fraction was methanol with an IC50 value of 161.16 μg/ml, all relative to 7.60 μg/ml of ascorbic acid. HPLC‑fingerprint analyses revealed that the major identified compounds in the ethyl acetate extract of C. elegans flowers are caffeic acid, coumaric acid, vanillin, and rutin, while for the n‑butanol extract ofC. nocturnum leaves are coumaric acid and vanillin. Conclusion:The obtained results revealed that the two species can be used as natural sources of antioxidant compounds with low cytotoxic effect on the mammalian cell line.

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      Nasr, S. M., Ghareeb, M. A., Mohamed, M. A., Elwan, N. M., Abdel-Aziz, A. E. A., & Abdel-Aziz, M. S. (). High‑Performance Liquid Chromatography‑Fingerprint Analyses, In vitro Cytotoxicity, Antimicrobial and Antioxidant Activities of the Extracts of Two Cestrum Species Growing in Egypt. Pharmacognosy Research, 10(2), 173–180. https://doi.org/10.4103/pr.pr_145_17