Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy ResearchNOW IN
    Article

    Mitigation of Radiation‑Induced Oxidative Stress by Methanolic Extract of Tragia involucrata in Swiss Albino Mice

    Nivya Machettira Thimmaiah1, Chandrashekhar Gajanan Joshi1★, Rajashekhar K Patil2, Ajay S Khandagale3, HM Somashekarappa4, D Ananda1, HM Manjunath1★ Corresponding author

    1. 1Department of Biochemistry, Mangalore University, Jnana Kavery, Chikka Aluvara, Kodagu, Karnataka, INDIA.
    2. 2Department of Applied Zoology, Mangalore University, Mangalore, Karnataka, INDIA.
    3. 3Department of Biochemistry, College of Fisheries, KVAFSU, Mangalore, Karnataka, INDIA.
    4. 4CARRT, Mangalore University, Mangalagangothri, Mangalore, Karnataka, INDIA.

    CORRESPONDENCE

    Chandrashekhar Gajanan Joshi

    Department of Biochemistry, Mangalore University, Jnana Kavery, Chikka Aluvara, Kodagu, Karnataka, INDIA.

    josheejoshee@gmail.com

    Volume 11, Issue 3 · pp. 236–243 · PUBLISHED · DOI: 10.4103/pr.pr_177_18

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Background: Tragia involucrata L. has been used in Indian traditional medicine since centuries to treat various ailments. The plant remains unexplored for its radioprotective properties and hence the present study. Objective: The main objective of the study is to ascertain the radioprotective effects of T. involucrata methanolic extract (TME) in Swiss albino mice against whole‑body gamma radiation. Materials and Methods: The acute toxicity of TME was evaluated. The optimal protective dose of TME against radiation‑induced mortality in mice was determined by survival analysis using the Kaplan–Meier curves. To understand the mechanism of radioprotection, TME was tested for hematological changes and antioxidant levels in mice by injecting100 mg/kg body weight intraperitoneally for 5 days and irradiated with a sublethal dose of 6 Gy gamma radiation. Results:The lethal dose 50%/of TME was about 645.65 mg/kgbw. The pretreatment of mice with 100 mg/kgbw of TME increased its survivability to 30% compared with the radiation control group and hence is considered as a radioprotective dose. The lethal dose 50%/30% of the irradiation alone group was found to be 9 Gy and 10.35 Gy for the TME + irradiation group. Hence, the dose reduction factor was about 1.15. The radiation decreased the hematological parameters in the blood, but on pretreatment with TME (100 mg/kgbw), increase in levels was noted. The antioxidant enzyme levels in the mice liver homogenate were found to be replenished in the test group compared with the radiation control. Conclusion:The present study indicates the protective role of TME against gamma radiation‑induced mortality and oxidative stress. Hence, it is proposed as a candidate for radioprotection.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Thimmaiah, N. M., Joshi, C. G., Patil, R. K., Khandagale, A. S., Somashekarappa, H., Ananda, D., & Manjunath, H. (). Mitigation of Radiation‑Induced Oxidative Stress by Methanolic Extract of Tragia involucrata in Swiss Albino Mice. Pharmacognosy Research, 11(3), 236–243. https://doi.org/10.4103/pr.pr_177_18