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    Chromatographic Profiles, Anti‑inflammatory, and Cytotoxicity Potential of Extracts of Banisteriopsis pubipetala (A. Juss)

    Jeane Ferreira Leal de Freitas1, Flávia Dayrell França2, Míriam Martins Chaves3, Elytania Veiga Menezes1, Afrânio Farias De Melo Júnior1, Dario Alves De Oliveira1, Clarice Avelar Almeida1, Kamylla Santos Teixeira1, Murilo Malveira Brandão1, Vanessa de Andrade Royo1★★ Corresponding author

    1. 1Department of General Biology, Montes Claros State University, Professor Darcy Ribeiro University Campus, Montes Claros, Minas Gerais, BRAZIL.
    2. 2Federal University of Minas Gerais, Institute of Biological Sciences, Belo Horizonte, Minas Gerais, BRAZIL.
    3. 3Federal University of Minas Gerais, Institute of Biological Sciences, Belo Horizonte, Minas Gerais; Federal University of Espírito Santo, North University Center of Espírito Santo, Sao Mateus, Espírito Santo, BRAZIL.

    CORRESPONDENCE

    Vanessa de Andrade Royo

    Department of General Biology, Montes Claros State University, Professor Darcy Ribeiro University Campus, Montes Claros, Minas Gerais, BRAZIL.

    vanroyo31@gmail.com

    Volume 11, Issue 4 · pp. 339–345 · PUBLISHED · DOI: 10.4103/pr.pr_71_19

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Background:Banisteriopsis pubipetala is a little‑studied species belonging to the same genus of B. caapi, which stands out for the bioactive compounds known to be important in the degenerative diseases treatment. Objective: The objective is to analyze the anti‑inflammatory and cytotoxic potential and chromatographic profile of the extracts of B. pubipetala.Materials and Methods:The investigation of the chromatographic profile was performed through high‑performance liquid chromatography diode‑array detector. Cell viability was determined by a quantitative colorimetric assay with 3‑(4,5‑dimethylthiazol‑2yl)‑2,5‑diphenyltetrazolium bromide. In the pharmacological tests, interleukin (IL)‑6, tumor necrosis factor‑alpha (TNF‑α), IL‑10, and nitric oxide (NO) levels in cell culture supernatants were performed. Results: In the leaf were observed nine major compounds and in the stem five major compounds. The extracts of B. pubipetala demonstrated concentration‑dependent behavior regarding cytotoxicity. The fraction in dichloromethane had inhibitory concentration of 50% (IC50) = 67.39 μg/mL whereas the extract in ethyl acetate had IC50 = 103.37 μg/mL. The results showed that extracts significantly reduced the production of IL‑6 and TNF‑α by cells 3T3 cells, however increased the production of IL‑10 and NO. Conclusion:The results of the tests indicate that the extracts of B. pubipetalaevaluated have potential anti‑inflammatory properties and may promote the regulation of inflammation levels.

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      Freitas, J. F. L. D., França, F. D., Chaves, M. M., Menezes, E. V., Júnior, A. F. D. M., Oliveira, D. A. D., Almeida, C. A., Teixeira, K. S., Brandão, M. M., & Royo, V. D. A. (). Chromatographic Profiles, Anti‑inflammatory, and Cytotoxicity Potential of Extracts of Banisteriopsis pubipetala (A. Juss). Pharmacognosy Research, 11(4), 339–345. https://doi.org/10.4103/pr.pr_71_19