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    Article

    Development of Chromatographic Fingerprint Profile and Multi-elemental Analysis of Datura metel L.

    Shakila Ramachandran1★, Saravanan Meenatchisundaram1, Subashini Subramanian1, Sujith Thatipelli1, Achintya Kumar Mandal1, Rajesh Allu1★ Corresponding author

    CORRESPONDENCE

    Shakila Ramachandran

    Received: 26-12-2021; Revised: 20-01-2022; Accepted: 15-02-2022.

    Volume 14, Issue 2 · pp. 146–152 · PUBLISHED · DOI: 10.5530/pres.14.2.21

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Background:The Solanaceae family member Datura metel L. has been related to therapeutic benefits. The plant is widely used in Ayurveda and Siddha systems of Indian medicines. Objectives: Present study was focused to understand the phytochemical and multi elemental composition of D. metelwhole plant. Materials and Methods: The whole plant of D. metel was collected, shade dried, powdered and subjected to analysis of physico-chemical parameters, thin layer chromatography, high performance thin layer chromatographic finger print profilings (HPTLC), high pressure liquid chromatographic profile at four wavelengths, X-ray fluorescence, energy dispersive X-ray analysis, X-ray photoelectron spectrometer, powder X-ray diffractometer and inductively coupled plasma optical emission spectrometer. Results: In HPTLC, 7 spots under 254 nm, 10 spots under 366 nm and 11 spots after derivatization with vanillin sulphuric acid reagent appeared. In HPLC, 9 peaks at 230 nm, 11 peaks at 254 nm, 5 peaks at 286 nm and 7 peaks at 366 nm separated. Presence of 17 elements such as copper, cobalt, nickel, manganese, zinc, iron, sodium, potassium, calcium, magnesium, phosphorus, aluminium, chlorine, carbon, oxygen, sulphur and silicon were identified. Conclusion:The elements K, Mg, Ca, Si, S and Fe play vital role on human health. Chlorine, silicon, sulfur and silica are first time identified which have significant biological functions.

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      Ramachandran, S., Meenatchisundaram, S., Subramanian, S., Thatipelli, S., Mandal, A. K., & Allu, R. (). Development of Chromatographic Fingerprint Profile and Multi-elemental Analysis of Datura metel L.. Pharmacognosy Research, 14(2), 146–152. https://doi.org/10.5530/pres.14.2.21