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    An HPLC Method to Determine Phenolic Compounds of Plant Extracts: Application to Byrsonima crassifolia and Senna alata Leaves

    Marília de A Cavalcante1★, Janyna dos S Oliveira1, Mayra S da S Barreto2, Lucas P Pinheiro2, Patrick de C Cantuária3, Wardsson L Borges4,5, Gabriel A da Silva2, Tiago Marcolino de Souza2★ Corresponding author

    1. 1Federal Institute of Amapá, Macapá, BRAZIL.
    2. 2State University of Amapá, Macapá, BRAZIL.
    3. 3Instituto de Pesquisas Científicas e Tecnológicas do Estado do Amapá, Macapá, BRAZIL.
    4. 4Embrapa Amapá, Macapá, BRAZIL.
    5. 5Embrapa Agroindústria Tropical, Fortaleza, BRAZIL.

    CORRESPONDENCE

    Marília de A Cavalcante

    Federal Institute of Amapá, Macapá, BRAZIL.

    marilia.cavalcante@yahoo. com.br

    Received: 22-08-2022; Revised: 29-09-2022; Accepted: 05-10-2022.

    Volume 14, Issue 4 · pp. 395–404 · PUBLISHED · DOI: 10.5530/pres.14.4.58

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Background:The Amazonian Region has a variety of medicinal plants with bioactive compounds, whose characterization could present the potential for sustainable development. Objectives:A method for separating, identifying, and quantifying a mixture of nine phenolic compounds (gallic acid, 3-hydroxybenzoic acid, p-coumaric acid, catechin, myricetin, rutin, quercetin, kaempferol, and cyanidin) was developed, validated, and applied to analyze aqueous and hydroethanolic extracts from Byrsonima crassifolia(L.) Kunth and Senna alata(L.) leaves. Materials and Methods:The separation was carried out by HPLC, using a Shim-pack VP-ODS C18 column (5 μm, 150 x 4.6 mm) at 40°C. Detection was performed at 254 nm and separation occurred in 35 min. Results:The optimized method was validated for each of the nine phenolic compounds. The calibration curve for the phenolic compound standards showed suitable linear fitting and exhibited correlation coefficients greater than 0.990. The LOD and LOQ varied between 6.2807 - 14.8851 μg mL-1 and 6.8002 - 16.0071 μg mL-1, respectively. The method was found to be robust for changes of ±2 ml in mobile phase composition. Byrsonima crassifoliaaqueous extracts indicated contents of gallic acid, catechin, rutin, and cyanidin whereas hydroethanolic one did not show the first substance. Senna alataaqueous extract presented only 3-hydroxybenzoic acid and rutin whereas myricetin, cyanidin, quercetin, and kaempferol were also identified in the hydroethanolic one. Conclusion:The HPLC method is efficient, precise, accurate, and sensitive to determining phenolic compounds in plant extracts and it is recommended for efficient assays in routine work.

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      Cavalcante, M. D. A., Oliveira, J. D. S., Barreto, M. S. D. S., Pinheiro, L. P., Cantuária, P. D. C., Borges, W. L., Silva, G. A. D., & Souza, T. M. D. (). An HPLC Method to Determine Phenolic Compounds of Plant Extracts: Application to Byrsonima crassifolia and Senna alata Leaves. Pharmacognosy Research, 14(4), 395–404. https://doi.org/10.5530/pres.14.4.58