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    Inhibitory Effect of Newbouldia laevis and Stereospermum acuminatissimum on Multi-Resistant Bacteria Isolates

    Jean Baptiste Hzounda Fokou1★, Nembu Collins Che Momfor1, Francine Kouemo2, Fru-Awah Akumawah1, Philippe Belle Ebanda Kedi3, Ruth Batindi1, Ornella Mpot1, David Tombe1, Lamana Dikehed1, Arnold Fomesseng Negoue1, Gisele Etame Loe3, Jules Clement Assob4★ Corresponding author

    1. 1Department of Pharmaceutical Sciences, Microbial Product Research Unit, Laboratory of Pharmacology, Faculty of Medicine and Pharmaceutical Sciences, Douala, CAMEROON.
    2. 2Department of Clinical Microbiology, Faculty of Health sciences, University of Buea, Buea, CAMEROON.
    3. 3Department of Pharmaceutical Sciences, Faculty of Medicine and Pharmaceutical Sciences, Douala, CAMEROON.
    4. 4Department of Biological Sciences Faculty of Medicine and Pharmaceutical Sciences, Douala, CAMEROON.

    CORRESPONDENCE

    Jean Baptiste Hzounda Fokou

    Department of Pharmaceutical Sciences, Microbial Product Research Unit, Laboratory of Pharmacology, Faculty of Medicine and Pharmaceutical Sciences, Douala, CAMEROON.

    hzoundafokou@gmail.com

    Received: 03-12-2023; Revised: 11-01-2024; Accepted: 16-02-2024.

    Volume 16, Issue 2 · pp. 331`–338 · PUBLISHED · DOI: 10.5530/pres.16.2.42

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Aim: Multi-drug-resistant bacteria is a growing concern worldwide. Plants have emerged as a promising avenue due to their bioactive compounds. Newbouldia laevis and Stereospermum acuminatissimumare two plants with a long history of use in traditional medicine. This study aimed at exploring the antimicrobial activity of their extracts against multi-drug-resistant bacteria. Materials and Methods: Extracts were prepared by maceration of pulverized leaves or stem barks (250 g) in 2L dichloromethane/methanol (30/70) filtered and the filtrate was dried using a rotary evaporator. The crude extracts were then tested against five pathogenic multi-resistant bacterial strains and Minimum Inhibition Concentration (MIC) was determined using the broth dilution method. The combinations of extracts and reference drug ceftriaxone was carried out following the checkerboard technique. Results:The overall results showed that Newbouldia laevis and Stereospermum acuminatissimumcrude extracts contain various phytochemicals such as alkaloids, flavonoids, terpenoids, and polyphenols, which were found to be associated with strong anti-bacterial effects against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumonia, exhibiting MICs values ranging from 100 μg/ mL to 400 μg/mL. The combinatorial analysis showed a combination and strains dependent-out, including synergism, additivity, and antagonism. Conclusion:These findings suggest that Newbouldia laevisandStereospermum acuminatissimumcould be used as alternative sources of antimicrobial agents for treating bacterial infections.

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      Fokou, J. B. H., Momfor, N. C. C., Kouemo, F., Akumawah, F., Kedi, P. B. E., Batindi, R., Mpot, O., Tombe, D., Dikehed, L., Negoue, A. F., Loe, G. E., & Assob, J. C. (). Inhibitory Effect of Newbouldia laevis and Stereospermum acuminatissimum on Multi-Resistant Bacteria Isolates. Pharmacognosy Research, 16(2), 331`–338. https://doi.org/10.5530/pres.16.2.42