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    Protective Effects of Vaccinium arctostaphylos L. against Oxymetholone-Injured Liver and Kidney Injury in BALB/c Mice: An Integrated Biochemical, Stereological, Histopathological, and Immunohistochemical Study

    Elnaz Khordad1, Mohsen Akbaribazm2,3★, Seyed Moein Hosseini4, Yasaman Rahmani4★ Corresponding author

    1. 1Department of Anatomical Sciences, School of Medicine, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, IRAN.
    2. 2Department of Anatomy, Torbat Heidariyeh University of Medical Sciences, Torbat Heidariyeh, IRAN.
    3. 3Department of Basic Medical Sciences, Khoy University of Medical Sciences, Khoy, IRAN.
    4. 4Student Research Committee, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, IRAN.

    CORRESPONDENCE

    Mohsen Akbaribazm

    Department of Anatomy, Torbat Heidariyeh University of Medical Sciences, Torbat Heidariyeh, IRAN.

    akbarim3@thums.ac.ir

    Received: 17-10-2023; Revised: 21-12-2023; Accepted: 16-02-2024.

    Volume 16, Issue 2 · pp. 401–413 · PUBLISHED · DOI: 10.5530/pres.16.2.50

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Background:Anabolic steroids, like Oxymetholone (OM), can imitate testosterone and cause harm to the liver and kidneys. Objectives:In this study, the ameliorative effects of Vaccinium arctostaphylosL. (V. arctostaphylos) on OM-induced liver and kidney damage were investigated using various methods. Materials and Methods:BALB/c mice (Thirty-six in 6 group/6 mice) were included as sham, OM, three OM groups treated with different doses of V. arctostaphylosfruit extract (OM+100, OM+200, and OM+400 V. arctostaphylos), and a group solely treated with 400 V. arctostaphylos. Blood samples collected on the 51st day assessed biochemical parameters and oxidative stress factors in the liver and kidney. Stereological and histopathological analyses were conducted using isolated liver and kidney tissues, while immunohistochemistry measured apoptosis levels using p53 and Bcl-2 positive cells. Results:The results indicated that V. arctostaphylosfruit extract protected the liver and kidney from OM-induced damage and maintained their normal function. Liver enzyme levels and kidney functional parameters were close to normal, and SOD and GPx activity increased while NO levels decreased. Stereological analysis showed that V. arctostaphylospreserved the normal structure of the liver and kidney and reduced the p53 positive cells while increasing Bcl-2 cell count. Conclusion:These findings suggest that V. arctostaphylosfruit extract, rich in polyphenols and possessing antioxidant properties, can enhance protection against OM-induced hepato-renal oxidative damage.

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      Khordad, E., Akbaribazm, M., Hosseini, S. M., & Rahmani, Y. (). Protective Effects of Vaccinium arctostaphylos L. against Oxymetholone-Injured Liver and Kidney Injury in BALB/c Mice: An Integrated Biochemical, Stereological, Histopathological, and Immunohistochemical Study. Pharmacognosy Research, 16(2), 401–413. https://doi.org/10.5530/pres.16.2.50