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    Article

    Exploring the Glycosidic Compounds in Ipomoea sagittifolia Stems: A High-Performance Liquid Chromatography Approach

    Raveesha Peeriga1★, Krishnaveni Manubolu2, Jayaprakash Somasundaram3, Sheikdawood Asanar Mohamed Shiek Arabath4, Shanthi Petchimuthu3, Sri Naga Joshitha Manepalli1, Vinay Kumar Maddela1, Kavya Makireddy1★ Corresponding author

    1. 1Department of Pharmacognosy, V. V. Institute of Pharmaceutical Sciences, Seshadri Rao Knowledge Village, Gudlavalleru, Krishna District, Andhra Pradesh, INDIA.
    2. 2Faculty of Pharmaceutical Sciences, Narayana Pharmacy College, Chinthareddypalem, Nellore, SPSR District, Andhra Pradesh, INDIA.
    3. 3Department of Pharmacy, K. M. College of Pharmacy, The Tamilnadu Dr. M. G. R. Medical University, Madurai, Tamil Nadu, INDIA.
    4. 4Department of Pharmacognosy, Arulmigu Kalasalingam College of Pharmacy, Anand Nagar, Krishnan Kovil, Tamil Nadu, INDIA.

    CORRESPONDENCE

    Raveesha Peeriga

    Department of Pharmacognosy, V. V. Institute of Pharmaceutical Sciences, Seshadri Rao Knowledge Village, Gudlavalleru, Andhra Pradesh, INDIA.

    drprsha@gmail.com

    Received: 04-10-2024; Revised: 02-11-11; Accepted: 03-12-2024.

    Volume 17, Issue 1 · pp. 162–172 · PUBLISHED 2025 · DOI: 10.5530/pres.20251964

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Background The abstract provides a comprehensive overview of the study on secondary metabolites from Ipomoea sagittifolia, emphasizing the extraction, isolation and characterization processes. Secondary metabolites, including alkaloids, flavonoids, phenols and glycosides, were identified and analyzed using various analytical techniques. Materials and Methods Ethanol extraction followed by Soxhlet apparatus yielded a crude extract, subsequently purified through column chromatography using a solvent mixture of n-butanol, glacial acetic acid and water. Thin Layer Chromatography (TLC) confirmed the presence of compounds across 108 eluted fractions, further validated by UV-vis spectroscopy revealing absorption peaks characteristic of glycosides at 288 nm. Results Fourier Transform Infrared (FTIR) spectroscopy highlighted functional groups such as alcohols, phenols, carboxylic acids and ketones, typical of glycoside structures. High-Performance Liquid Chromatography (HPLC) confirmed the presence of cardiac glycosides through retention time analysis, aligning closely with Scillaren A standards. Conclusion These findings underscore the pharmacological potential of Ipomoea sagittifolia in traditional medicine and pharmaceutical applications, supported by its diverse secondary metabolite profile. Future research could delve deeper into bioactivity assays to elucidate specific therapeutic properties linked to these metabolites, thereby enhancing its relevance in healthcare and biotechnological innovations.

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      Peeriga, R., Manubolu, K., Somasundaram, J., Arabath, S. A. M. S., Petchimuthu, S., Manepalli, S. N. J., Maddela, V. K., & Makireddy, K. (2025). Exploring the Glycosidic Compounds in Ipomoea sagittifolia Stems: A High-Performance Liquid Chromatography Approach. Pharmacognosy Research, 17(1), 162–172. https://doi.org/10.5530/pres.20251964