Evaluation of Neuroprotective Potential of Water-Dispersible Turmeric Extract and Sustained-Release Ashwagandha Root Extract against B[A]P Induced Neurotoxicity in Zebrafish

ABSTRACT

Background:Numerous advantages of using herbs in the management of neurodegenerative disorders have been documented. Water-Dispersible Turmeric Extract Containing 60% Natural Curcuminoids (WDTE60N) and Sustained-Release Ashwagandha Root Extract (AshwaSR) are the herbal formulations prepared from the extracts of turmeric rhizome and Ashwagandha root, respectively. Benzo[a]pyrene (B[a]P), a polycyclic aromatic hydrocarbon, has recently emerged as a neurotoxic compound that impacts zebrafish behaviour and triggers oxidative stress. This neurotoxic effect illustrates the link between oxidative stress and neurodegeneration, as well as the development of anxiety-like behaviours in zebrafish. This study aimed to investigate the neuroprotective potential of WDTE60N and AshwaSR against B[a]P-induced neurotoxicity in zebrafish. Materials and Methods:In geometric sequence, 5 different concentrations of each formulation were prepared and administered to zebrafish for 7 days via bath immersion technique. Before the administration of active concentration, fish were exposed to B[a]P for seven days. Neurobehavioral patterns were assessed by using novel tank diving test and light and dark box test. Results: Exposure to B[a]P for seven days reduced the locomotor activity of zebrafish and increased their preference for the lighter part of the glass tank. A notable increase in locomotor activity and higher preference for diving towards lower zone of tank was observed in the zebrafish treated with WDTE60N or AshwaSR. The histopathological findings showed that supplementation with these herbal formulations resulted in reduction in congestion of the corpus cerebella region of adult zebrafish brain. Conclusion: Collectively, WDTE60N and AshwaSR demonstrated neuroprotective potential against B[a]P-induced neurobehavioral alterations in the zebrafish.

KEYWORDS

• Ashwagandha
• B[a]P
• Neurodegeneration
• Neurobehavioral
• Turmeric
• Zebrafish

Showing references and in-text citations exactly as published.

• DOI10.5530/pres.20252280Copy

RELATED ARTICLES

Matched on section and article type within this issue of Pharmacogn. Res..

• Article · VOLUME 17, ISSUE 4Comparative LC-MS Profiling of two Linderniaceae SpeciesThachan Sreelakshmi, Meethaley Valappil Jeeshna, Abhijith Sarga, Sugunan Ashitha · pp. 1187–1196Original ArticleSame type: Article
• Article · VOLUME 17, ISSUE 4Investigating the Expression of Apoptosis and Metastasis Genes in Satureja khuzistanica along with Nisin and Doxorubicin in Colorectal CancerEsmaeil Kheiri, Zahra Hojjati Bonab, Hossein Soltanzadeh · pp. 1197–1204Original ArticleSame type: Article
• Article · VOLUME 17, ISSUE 4Nephroprotective and Cytotoxic Assessment of Moringa concanensis Leaf Extract in Gentamicin-Induced Renal Injury in RatsAbhishek Raghavendra, Hariprasad Madakasira Guggilla, Diya Lakshman Shetty, Deeksha Giridhar · pp. 1205–1213Original ArticleSame type: Article
• Article · VOLUME 17, ISSUE 4Evaluation of Anti-Depressant Activity of Garcinia cambogia Fruit Using Animal ModelsJ. Priyadharisini, N. Savitha, Lydia Jeeboi Paramel · pp. 1214–1217Original ArticleSame type: Article