Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy ResearchNOW IN
    Article

    Chemical constituents and bioactivities of Glinus oppositifolius

    Consolacion Y. Ragasa1,2★, Esperanza C. Cabrera3, Oscar B. Torres2, Adiel Inah Buluran2, Dinah L. Espineli2, Dennis D. Raga4, Chien-Chang Shen5★ Corresponding author

    1. 1Department of Chemistry, De La Salle University Science and Technology Complex Leandro V. Locsin Campus, Biñan City, Laguna 4024
    2. 2Departments of Chemistry
    3. 3Biology, De La Salle University, 2401 Taft Avenue, Manila 1004
    4. 4Department of Biology, School of Science and Engineering, Ateneo de Manila University, Loyola Heights, Quezon City 1108, Philippines
    5. 5Division of Chinese Medicinal Chemistry, National Research Institute of Chinese Medicine, Ministry of Health and Welfare, 155-1, Li-Nong St., Sec. 2, Taipei 112, Taiwan

    CORRESPONDENCE

    Consolacion Y. Ragasa

    *Address for correspondence: Dr. Consolacion Y. Ragasa, Department of Chemistry, De La Salle University Science and Technology Complex Leandro V. Locsin Campus, Biñan City, Laguna 4024, Philippines, De La Salle University, 2401 Taft Avenue, Manila 1004, Philippines.

    consolacion.ragasa@dlsu.edu.ph

    Revised: 03-11-2014.

    Volume 7, Issue 2 · pp. 138–147 · PUBLISHED April-June 2015 · DOI: 10.4103/0974-8490.150520

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Objectives To isolate the secondary metabolites from the dichloromethane (DCM) extracts of Glinus oppositifolius; to test for the cytotoxicity of a new triterpene, oppositifolone (1); and to test for the hypoglycemic, analgesic, and antimicrobial potentials of 1, DCM and aqueous leaf extracts of G. oppositifolius. Methods The compounds were isolated by silica gel chromatography and identified by nuclear magnetic resonance spectroscopy. The cytotoxicity potential of 1 was tested using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Triterpene 1, DCM, and aqueous leaf extracts were tested for hypoglycemic potential using the oral glucose tolerance test; analgesic potential using the tail-flick assay, and antimicrobial potential using the disc diffusion method. Results The DCM extracts of G. oppositifolius afforded 1, squalene, spinasterol, oleanolic acid, phytol, and lutein from the leaves; squalene and spergulagenin A from the stems; and spinasterol from the roots. Triterpene 1 was cytotoxic against human colon carcinoma 116 with an IC50 value of 28.7 but did not exhibit cytotoxicity against A549. The aqueous leaf extract at 200 mg/kg body weight (BW) exhibited hypoglycemic activity with a pronounced % blood glucose reduction of 70.76% ±17.4% within 0.5 h after introduction. The DCM leaf extract showed a lower % blood glucose reduction of 18.52% ±13.5% at 200 mg/kg BW within 1.5 h after introduction, while 1 did not exhibit hypoglycemic activity. The samples did not exhibit analgesic property and were inactive against multiple drug resistant bacterial pathogens. Conclusion The compounds responsible for the hypoglycemic activity of G. oppositifolius which are fast acting (0.5 h) are found in the aqueous leaf extract.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Ragasa, C. Y., Cabrera, E. C., Torres, O. B., Buluran, A. I., Espineli, D. L., Raga, D. D., & Shen, C. (2015). Chemical constituents and bioactivities of Glinus oppositifolius. Pharmacognosy Research, 7(2), 138–147. https://doi.org/10.4103/0974-8490.150520