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    Pharmacognosy ResearchNOW IN
    Article

    Chemopreventive effect of methanolic extract of Azadirachta indica on experimental Trypanosoma brucei induced oxidative stress in dogs

    Temidayo O. Omobowale1★, Ademola A. Oyagbemi1, Oyefunbi A. Oyewunmi1, Olumuyiwa A. Adejumobi1★ Corresponding author

    1. 1Departments of Veterinary Medicine and Physiology, Biochemistry and Pharmacology, University of Ibadan, Ibadan, Nigeria

    CORRESPONDENCE

    Temidayo O. Omobowale

    *Address for correspondence: Dr. Temiidayo O. Omobowale, Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Ibadan, Nigeria. ; bukitayo_omobowale@yahoo.com

    omobowale@mail.ui.edu.ng

    Revised: 08-01-2015.

    Volume 7, Issue 3 · pp. 249–258 · PUBLISHED July-September 2015 · DOI: 10.4103/0974-8490.157972

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Introduction The medicinal properties of Azadirachta indica have been harnessed for many years in the treatment of many diseases in both humans and animals. Materials and Methods Twenty-five apparently healthy dogs weighing between 3 and 8 kg were randomly divided into five groups with five dogs in each group. Ameliorative effect of A. indica on erythrocyte antioxidant status and markers of oxidative stress were assessed. Liver and kidney function tests were also performed. Results Pre-treatment with methanolic extract of Azadirachta indica (MEAI) at different doses did not significantly alter the values of alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase activity in Trypanosoma brucei infection. Although, serum creatinine significantly (P < 0.05) decreased with pre-treatment with 50 mg/kg A. indica, after 2 weeks of T. brucei infection. However, the reduced glutathione (GSH) content of the erythrocyte increased significantly in animals pre-treated with 50 mg/kg and 200 mg/kg of A. indica respectively. Markers of oxidative stress such as malondialdehyde and hydrogen peroxide generated were higher in animals infected with T. brucei with no significant (P >0.05) difference compared to the values obtained in pre-treated animals. Pre-treatment with 100 mg/kg and 200 mg/kg of A. indica significantly (P < 0.05) decreased serum myeloperoxidase activity at 2 weeks post-infection with T. brucei. Conclusion From this study, MEAI showed significant ability to attenuate oxidative stress and inflammation during experimental T. brucei infection.

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      Omobowale, T. O., Oyagbemi, A. A., Oyewunmi, O. A., & Adejumobi, O. A. (2015). Chemopreventive effect of methanolic extract of Azadirachta indica on experimental Trypanosoma brucei induced oxidative stress in dogs. Pharmacognosy Research, 7(3), 249–258. https://doi.org/10.4103/0974-8490.157972