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    Quantification of Quercetin and Rutin from Benincasa hispida Seeds and Carissa Congesta Roots by High-performance Thin Layer Chromatography and High-performance Liquid Chromatography

    Gaurav Mahesh Doshi1,2★, Hemant Devidas Une3★ Corresponding author

    1. 1Department of Pharmacology, Vivekanand Education Society’s College of Pharmacy, Mumbai, Maharashtra
    2. 2Ph.D. Research Scholar, Pacific Academy of Higher Education and Research University, Udaipur, Rajasthan
    3. 3Department of Pharmacology, Y. B. Chavan College of Pharmacy, Rouzabagh, Aurangabad, Maharashtra, India

    CORRESPONDENCE

    Gaurav Mahesh Doshi

    Department of Pharmacology, Vivekanand Education Society’s College of Pharmacy, Mumbai, Maharashtra, India.

    gaurav.pharmacology@gmail.com

    Volume 8, Issue 1 · pp. 37–42 · PUBLISHED Jan-Mar 2016 · DOI: 10.4103/0974-8490.171098

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Objective In Indian Ayurvedic system, Benincasa hispida (BH) and Carissa congesta (CC) are well-known plants used for major and minor ailments. BH has been regarded as Kushmanda, whereas CC has been used in immune-related disorders of the human system. Quercetin and rutin identified from the vast plethora of plant extracts have proved to possess ethnopharmacological relevance. Materials and Methods In present studies, we have determined quercetin and rutin in terms of percentage in BH seeds and CC roots by high-performance thin layer chromatography (HPTLC) and high-performance liquid chromatography (HPLC). After extraction and phytochemical screening, the extracts were subjected to quantification for the presence of quercetin and rutin by HPTLC and HPLC. Results HPTLC showed quercetin as 44.60, 27.13% and rutin as 32.00, 36.31% w/w, whereas HPLC revealed quercetin as 34.00, 35.00% and rutin as 21.99, 45.03% w/v in BH and CC extracts, respectively. Conclusion The BH and CC extracts have elucidated peaks that were corresponding with standard peaks on undertaking chromatographic studies.

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      Doshi, G. M., & Une, H. D. (2016). Quantification of Quercetin and Rutin from Benincasa hispida Seeds and Carissa Congesta Roots by High-performance Thin Layer Chromatography and High-performance Liquid Chromatography. Pharmacognosy Research, 8(1), 37–42. https://doi.org/10.4103/0974-8490.171098