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    Evaluation of In Vitro Cytotoxic and Antioxidant Activity of Datura metel Linn. and Cynodon dactylon Linn. Extracts

    Soumen Roy1★, Sandip Pawar2, Abhay Chowdhary1★ Corresponding author

    1. 1Department of Virology, Ha~kine Institute for Training, Research and Testing, Parel
    2. 2Department of Biochemistry, The Institute of Science, Fort, Mumbai, Maharashtra, India

    CORRESPONDENCE

    Soumen Roy

    Department of Virology, Haffkine Institute for Training, Research and Testing, Parel, Mumbai, Maharashtra, India.

    soumenroy118@gmail.com

    Volume 8, Issue 2 · pp. 123–127 · PUBLISHED Apr-Jun 2016 · DOI: 10.4103/0974-8490.175610

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Aim To evaluate in vitro cytotoxicity and antioxidant activity of Datura metel L. and Cynodon dactylon L. extracts. Materials and Methods The extraction of plants parts (datura seed and fruit pulp) and areal parts of durva was carried out using soxhlet and cold extraction method using solvents namely methanol and distilled water. The total phenolic content (TPC) and total flavonoid content (TFC) was determined by established methods. The in vitro cytotoxicity assay was performed in vero cell line by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay method. In vitro antioxidant activity of the extract was performed by 2, 2-diphenyl-1-picrylhydrazyl radical scavenging method. Results We found that the highest amount of TPC and TFC in methanolic extracts of seed (268.6 mg of gallic acid equivalence/mg of dry plant material) and fruit pulp (8.84 mg of quercetin equivalence/mg dry plant material) of D. metel, respectively prepared by Soxhlet method. The methanolic extract of C. dactylon prepared using soxhlation has shown potent free radical scavenging activity with 50% inhibitory concentration (IC50) value of 100 mg/ ml. The IC50 of a methanolic cold extract of datura fruit was found to be 3 mg/ml against vero cell line. Conclusion We observed that plant parts of C. dactylon and D. metel have a high antioxidant activity. Further research is needed to explore the therapeutic potential of these plant extracts.

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      Roy, S., Pawar, S., & Chowdhary, A. (2016). Evaluation of In Vitro Cytotoxic and Antioxidant Activity of Datura metel Linn. and Cynodon dactylon Linn. Extracts. Pharmacognosy Research, 8(2), 123–127. https://doi.org/10.4103/0974-8490.175610