Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy ResearchNOW IN
    Article

    Anti-inflammatory Activity of Berry Fruits in Mice Model of Inflammation is Based on Oxidative Stress Modulation

    Geisson Marcos Nardi1, Adriana Graziele Farias Januario1, Cassio Geremia Freire2, Fernanda Megiolaro2, Kétlin Schneider2, Marlene Raimunda Andreola Perazzoli2, Scheley Raap Do Nascimento1, Ana Cristina Gon3, Luísa Nathália Bolda Mariano3, Glauber Wagner4, Rivaldo Niero3, Claudriana Locatelli2★★ Corresponding author

    1. 1Laboratory of Pharmacology, Life Sciences Branch, University of the West of Santa Catarina – Unoesc
    2. 2Post Graduate Program in Science and Biotechnology, Branch of Biotechnology, University of the West of Santa Catarina – Unoesc
    3. 3Post Graduate Program in Pharmaceutical Sciences and Chemical-Pharmaceutical Investigations Center, University of Itajaí Valley - Univali
    4. 4Laboratory of Infectious and Parasitic Diseases, Life Sciences Branch, University of the West of Santa Catarina – Unoesc, Brazil

    CORRESPONDENCE

    Claudriana Locatelli

    Universidade do Oeste de Santa Catarina, Núcleo Biotecnológico, Bloco K - 1º andar - Paese St., Videira, CEP: 89560-000, Santa Catarina, Brazil.

    claudriana.locatelli@unoesc.edu.br, claudrilocatelli@gmail.com

    Volume 8, Issue 5S · pp. S42–S49 · PUBLISHED 2016 · DOI: 10.4103/0974-8490.178642

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Background Many fruits have been used as nutraceuticals because the presence of bioactive molecules that play biological activities. Objective The present study was designed to compare the anti-inflammatory and antioxidant effects of methanolic extracts of Lycium barbarum (GOJI), Vaccinium macrocarpon (CRAN) and Vaccinium myrtillus (BLUE). Materials and Methods Mices were treated with extracts (50 and 200 mg/kg, p.o.), twice a day through 10 days. Phytochemical analysis was performed by high-performance liquid chromatography. Antioxidant activity was determine by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, reducing power, lipid peroxidation thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH) and catalase (CAT) activity. Anti-inflammatory activity was evaluated by paw edema followed by determination of myeloperoxidase (MPO) and TBARS. Results High amount of phenolic compounds, including rutin, were identified in all berries extracts. However, quercetin was observed only in BLUE and CRAN. GOJI presents higher scavenging activity of DPPH radical and reducing power than BLUE and CRAN. The extracts improved antioxidant status in liver; BLUE showed the largest reduction (75.3%) in TBARS when compared to CRAN (70.7%) and GOJI (65.3%). Nonetheless, CAT activity was lower in BLUE group. However, hepatic concentrations of GSH were higher in animals treated with GOJI rather than CRAN and BLUE. Despite all fruits caused a remarkable reduction in paw edema and TBARS, only BLUE and CRAN were able to reduce MPO. Conclusion These results suggest that quercetin, rutin, or other phenolic compound found in these berry fruits extracts could produce an anti-inflammatory response based on modulation of oxidative stress in paw edema model.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Nardi, G. M., Januario, A. G. F., Freire, C. G., Megiolaro, F., Schneider, K., Perazzoli, M. R. A., Nascimento, S. R. D., Gon, A. C., Mariano, L. N. B., Wagner, G., Niero, R., & Locatelli, C. (2016). Anti-inflammatory Activity of Berry Fruits in Mice Model of Inflammation is Based on Oxidative Stress Modulation. Pharmacognosy Research, 8(5S), S42–S49. https://doi.org/10.4103/0974-8490.178642