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    Extracts from Flammulina velutipes Inhibit the Adhesion of Pathogenic Fungi to Epithelial Cells

    Svetlana Kashina1, Lérida Liss Flores Villavicencio1, Marco Balleza2, Gloria Barbosa Sabanero3, Víctor Tsutsumi4, Myrna Sabanero López1★★ Corresponding author

    1. 1Department of Biology, Division of Natural and Exact Sciences, Campus Guanajuato, University of Guanajuato, Noria Alta S/N, Col. Noria Alta, Guanajuato, Guanajuato 36050
    2. 2Department of Science and Engineering, Campus León, University of Guanajuato, Loma del Bosque 103 Col, Lomas del Campestre, León, Guanajuato 37150
    3. 3Department of Medical Sciences, Division of Health Sciences, Campus León, University of Guanajuato, 20 de Enero 292, Col. Obregón, León, Guanajuato 37320
    4. 4Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies, National Polytechnic Institute (CINVESTAV-IPN), Av. Instituto Politécnico Nacional 2508, San Pedro Zacatenco, Gustavo A, Madero, 07360, México City, México

    CORRESPONDENCE

    Myrna Sabanero López

    Dr. Myrna Sabanero Lopez, Department of Biology, Division of Natural and Exact Sciences, Campus Guanajuato, University of Guanajuato, Noria Alta S/N, Col. Noria Alta, Guanajuato.

    myrna.sabanero@gmail.com

    Volume 8, Issue 5S · pp. S56–S60 · PUBLISHED 2016 · DOI: 10.4103/0974-8490.178648

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Background Recently, extracts from natural sources have been tested for their antifungal properties. In this aspect, Flammulina velutipes extracts possess a significant amount of branch-chained carbohydrates with mannose moieties that, hypothetically, can reduce the adhesion. Objective In this study, we assessed the capacity of extracts from F. velutipes (wild-type AQF-1 and ATCC 34574 as the reference strain) to inhibit the adhesion of S. schenkii and C. albicans to epithelial cells. Materials and Methods The aqueous extracts from F. velutipes strains were obtained by sonication, total carbohydrate and protein was analyzed by Dubois and Lowry methods respectively. Effect of the extracts (50, 100 and 150 mg/mL) on the fungi adhesion to host cells was evaluated after 1 h interaction, and the percentage of inhibition of adhesion was measured. After of interaction the cytoskeleton from cell was analyzed with phalloidin-FITC. Results The extract from strain AQF-1 (50, 100 and 150 mg/mL) inhibited the adhesion of: S. schenkii in a dose-dependent manner (4.9, 7.5 and 12.7%, respectively) and C. albicans in a dose-independent manner (5.2%). The percentage of inhibition by extracts from the strain ATCC34574 at the same concentrations, shown that are dose independent for both fungi: 3.9% for S. schenkii and 2.6% for C. albicans. Conclusion The extracts from F. velutipes inhibit the adhesion of pathogenic fungi to host cells. The mechanism molecular is unknown; however, is probably an interaction between the polysaccharides from extracts with the fungi receptors. This aspect is currently analyzed.

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      Kashina, S., Villavicencio, L. L. F., Balleza, M., Sabanero, G. B., Tsutsumi, V., & López, M. S. (2016). Extracts from Flammulina velutipes Inhibit the Adhesion of Pathogenic Fungi to Epithelial Cells. Pharmacognosy Research, 8(5S), S56–S60. https://doi.org/10.4103/0974-8490.178648