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    Antioxidant and Intracellular Reactive Oxygen Species/Reactive Nitrogen Species Scavenging Activities of Three Porcupine Bezoars from Hystrix brachyura

    Peng Nian Yew1, Wai Leng Lee1, Yau Yan Lim1★★ Corresponding author

    1. 1School of Science, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, 47500 Subang Jaya, Selangor, MALAYSIA.

    CORRESPONDENCE

    Yau Yan Lim

    School of Science, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, 47500 Subang Jaya, Selangor, MALAYSIA.

    lim.yau.yan@monash.edu

    Volume 9, Issue 4 · pp. 366–371 · PUBLISHED · DOI: 10.4103/pr.pr_145_16

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Background:Porcupine dates are phytobezoar stones that are used in Traditional Chinese Medicine (TCM) treatments against cancer, postsurgical recovery, dengue fever, etc. The medicinal values have not been scientifically investigated due to the availability and high pricing of the dates. Objectives:This paper represents the first report on the phytochemical content, in vitro antioxidant and intracellular reactive oxygen species (ROS)/reactive nitrogen species (RNS) scavenging properties of the extracts of three porcupine dates: grassy date (GD), black date (BD), and powdery date (PD). Materials and Methods:Dried samples were extracted with methanol and lyophilized. Samples were screened for phytochemical constituents, in vitroantioxidant assays based on total phenolic content (TPC), free radical scavenging, and ferric reducing power (FRP) as well as intracellular ROS and RNS scavenging properties. Results:Phytochemical screening and total tannins assay revealed that tannins, cardiac glycosides, and terpenoids were found in all porcupine dates with tannins forming the major portion of the TPC. In comparison to GD, BD and PD were found to contain significantly high TPC, radical scavenging activity, and FRP. At 200 µg/ml, BD and PD remarkably scavenged 2, 2‑azobis (2‑amidinopropane) dihydrochloride‑induced ROS in RAW264.7 cells and significantly reduced nitric oxide in lipopolysaccharide‑stimulated cells. Conclusion: Overall, BD and PD exhibited promising in vitroantioxidant as well as intracellular ROS/RNS scavenging properties.

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      Yew, P. N., Lee, W. L., & Lim, Y. Y. (). Antioxidant and Intracellular Reactive Oxygen Species/Reactive Nitrogen Species Scavenging Activities of Three Porcupine Bezoars from Hystrix brachyura. Pharmacognosy Research, 9(4), 366–371. https://doi.org/10.4103/pr.pr_145_16