Identification and Characterization of Memecylon Species using Isozyme Profiling
TR Bharathi1, Shailasree Sekhar2, N Geetha1, SR Niranjana3, HS Prakash1★★ Corresponding author
- 1Department of Studies in Biotechnology, University of Mysore, Mysore, INDIA.
- 2Institution of Excellence, Vijnana Bhavana, University of Mysore, Mysore, INDIA.
- 3Vice Chancellor, Gulbarga University, Kalaburagi, Karnataka, INDIA.
CORRESPONDENCE
HS Prakash
Department of Studies in Biotechnology, University of Mysore, Mysore, INDIA.
Volume 9, Issue 4 · pp. 408–413 · PUBLISHED · DOI: 10.4103/0974-8490.218499
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ABSTRACT
Background:The protein/isozyme fingerprint is useful in differentiating the species and acts as a biochemical marker for identification and systematic studies of medicinal plant species. Objective: In the present study, protein and isozyme profiles for peroxidase, esterase, acid phosphatase, polyphenol oxidase, alcohol dehydrogenase, and alkaline phosphatase of five species of Memecylon (Melastomataceae), Memecylon umbellatum, Memecylon edule, Memecylon talbotianum, Memecylon malabaricum, and Memecylon wightii were investigated. Materials and Methods:Fresh leaves were used to prepare crude enzyme extract for analyzing the five enzymes isozyme variations. Separation of isozymes was carried out using polyacrylamide gel electrophoresis (PAGE) and the banding patterns of protein were scored. Pair-wise comparisons of genotypes, based on the presence or absence of unique and shared polymorphic products, were used to regenerate similarity coefficients. The similarity coefficients were then used to construct dendrograms, using the unweighted pair group method with arithmetic averages. Results: A total of 50 bands with various Rf values and molecular weight were obtained through PAGE analysis. Among the five Memecylonspecies, more number of bands was produced in M. wightii and less number of bands was observed in M. edule. The results of similarity indices grouped M. malabaricum and M. wightii in one cluster with 98% similarity and M. umbellatum, M. edule, and M. talbotianum are grouped in another cluster with 79% similarity showing close genetic similarities which is in accordance with the morphological identification of Memecylon species. Conclusion: The protein/isozyme fingerprint is useful in differentiating the species and acts as a biochemical marker for identification ofMemecylon species.
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Bharathi, T., Sekhar, S., Geetha, N., Niranjana, S., & Prakash, H. (). Identification and Characterization of Memecylon Species using Isozyme Profiling. Pharmacognosy Research, 9(4), 408–413. https://doi.org/10.4103/0974-8490.218499