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    In vitro Antiproliferative Effect of Earthworm Coelomic Fluid of Eudrilus Eugeniae, Eisenia foetida, and Perionyx Excavatus on Squamous Cell Carcinoma‑9 Cell Line: A Pilot Study

    Dominic Augustine2★, Roopa S. Rao2, Jayaraman Anbu2, K. N. Chidambara Murthy3★ Corresponding author

    1. 1Department of Oral Pathology and Microbiology, Faculty of Dental Sciences, M. S. Ramaiah University of Applied Sciences,.
    2. 2Department of Pharmacology, Faculty of Pharmacy, M. S. Ramaiah University of Applied Sciences.
    3. 3Central Research Laboratory, M. S. Ramaiah Medical College and Hospital, Bengaluru, Karnataka, India.

    CORRESPONDENCE

    Dominic Augustine

    dominic2germain@gmail.com

    Volume 9, Issue 5S · pp. s61–s66 · PUBLISHED · DOI: 10.4103/pr.pr_52_17

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Introduction:The earthworm coelomic fluid (ECF) has shown proven antiproliferative effect against breast, liver, gastrointestinal, and brain cancer, but it is least explored in oral cancer. The presentin vitrostudy is an attempt to investigate the antiproliferative activity of ECF on oral cancer cell line squamous cell carcinoma (SCC)-9.Materials and Methods:ECF was collected from the speciesEudrilus eugeniae(EE),Eisenia foetida(EF), andPerionyx excavatus(PE) stored at −80°C. Percentage inhibition of ECF on squamous cell carcinoma-9 cellsin vitrowas recorded at 24 h. Protein estimation was done using Bradford protein assay validated by the biuret method. Cytotoxicity was tested at 2.5, 5, 10, 20, 40, and 80 μg/ml concentrations by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay in SCC-9 cellsin vitro.GraphPad Prism 7.0 software was used to calculate the inhibitory concentration (IC50). Chi-square test was used to analyze the difference between samples.Results:The test samples EE, EF, and PE inhibited the growth of SCC-9 cells significantly in a dose-dependent manner, and the IC50values were found to be 4.6, 44.69, and 5.27 μg/ml, respectively. The antiproliferative effect was found to be variable among the three earthworm species with EE showing the most promising effect followed by PE and EF.Conclusion:Establishing the antiproliferative effect of ECF on oral cancer cells could be an initial step toward drug development and future anticancer research. The preliminary investigation has shown that ECF has a promising antiproliferative effect on oral cancer cellsin vitro.

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      Augustine, D., Rao, R. S., Anbu, J., & Murthy, K. N. C. (). In vitro Antiproliferative Effect of Earthworm Coelomic Fluid of Eudrilus Eugeniae, Eisenia foetida, and Perionyx Excavatus on Squamous Cell Carcinoma‑9 Cell Line: A Pilot Study. Pharmacognosy Research, 9(5S), s61–s66. https://doi.org/10.4103/pr.pr_52_17