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    Free Radicals and AntioxidantsNOW IN
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    Photochemoprotective Effects of Ethyl Acetate Fraction from Senegalia polyphylla Leaves in Ultraviolet-Irradiated L929 Fibroblasts

    Regina Gomes Daré1, Lilian dos Anjos Oliveira Ferreira1, Fabianne Martins Ribeiro1, Jesuí Vergilio Visentainer2, Celso Vataru Nakamura3, Maria da Conceição Torrado Truiti1★★ Corresponding author

    1. 1Departamento de Farmácia, Universidade Estadual de Maringá, Maringá, Paraná, BRAZIL.
    2. 2Departamento de Química, Universidade Estadual de Maringá, Maringá, Paraná, BRAZIL.
    3. 3Departamento de Ciências Básicas da Saúde, Universidade Estadual de Maringá, Paraná, BRAZIL.

    CORRESPONDENCE

    Maria da Conceição Torrado Truiti

    Departamento de Farmácia, Universidade Estadual de Maringá, Maringá, Paraná, BRAZIL.

    mcttruiti@uem.br

    Received: 03-05-2021; Revised: 21-06-2021; Accepted: 05-07-2021.

    Volume 11, Issue 1 · pp. 7–12 · PUBLISHED · DOI: 10.5530/fra.2021.1.2

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    ABSTRACT

    Objectives: Unprotected exposure to ultraviolet radiation causes oxidative damages to skin cells. Topical administration of antioxidants is a feasible strategy to prevent oxidative alterations. Therefore, the present study evaluated the photodamage attenuating potential of plant materials from Senegalia polyphylla leaves, due to previous studies relating Senegalia species as a source of antioxidant phenolic compounds. Materials and Methods: The ethanolic extract (EE) and its ethyl acetate (EAF), hydromethanolic and hexane fractions were evaluated for their total phenolic content and antioxidant capacity. The photochemoprotective effects of plant materials with higher antioxidant potential were assessed in L929 fibroblasts against ultraviolet-B (UVB) and Ultraviolet-A (UVA) radiations. Phytochemical investigation of bioactive plant material was performed and compounds identified by nuclear magnetic resonance analysis. Results: The EE and EAF presented the highest total phenolic content and antioxidant capacity, showing ferric reducing power and ability to scavenge free radicals DPPH•, ABTS•+, O2•− and ROO•. The EE and EAF treatments prior to UVB and UVA irradiation prevented the decrease in cell viability, and attenuated reactive oxygen species generation, reduced glutathione depletion, lipid peroxidation and plasma membrane disruption, especially with EAF treatment. Vitexin and isoquercetin, known antioxidant compounds, were isolated from EAF, which may be correlated with its photochemoprotective ability. Conclusion: Findings indicate the potential of polyphenol-enriched botanical materials, such as EAF from S. polyphylla, in preventing UVB and UVA-induced oxidative damages, due to its effective antioxidant activity and ability to attenuate redox imbalance and reduce cell damages.

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      Daré, R. G., Ferreira, L. D. A. O., Ribeiro, F. M., Visentainer, J. V., Nakamura, C. V., & Truiti, M. D. C. T. (). Photochemoprotective Effects of Ethyl Acetate Fraction from Senegalia polyphylla Leaves in Ultraviolet-Irradiated L929 Fibroblasts. Free Radicals and Antioxidants, 11(1), 7–12. https://doi.org/10.5530/fra.2021.1.2