Methaemoglobin and Malondialdehyde Levels of Human Erythrocytes Incubated with Acetylsalicylic Acid

ABSTRACT

Background:MetHb and MDA are biomarkers for erythrocyte redox status and membrane lipid peroxidation, respectively. This present study aims to investigate the levels of human erythrocyte redox status and membrane lipid peroxidation indicators in the presence of ASA over time using in vitro models. Materials and Methods: The %MetHb was evaluated by measuring the absorbance of human erythrocyte suspension at λmax=630 nm and 540 nm. Human erythrocytes were incubated in 0.125 mg/mL, 0.25 mg/mL and 0.5 mg/mL ASA over a 6 hr incubation period. The human erythrocyte MDA concentration was measured using the TBARS assay. Results: The %MetHb of human erythrocytes following 3 hr of incubation with 0.125 mg/mL ASA gave a peak value of 2.70±1.36%, indicating a relative significant (p<0.05) increase of 45.16% compared with the control samples. Furthermore, an increase in the incubation time resulted in a decrease in erythrocyte %MetHb, although not to the levels observed at the beginning (t=0 hr) of incubation with ASA. Conversely, MDA concentration of human erythrocytes was 4.45±0.89 μM after 3 hr of incubation with 0.125 mg/mL ASA, showing a significant (p<0.05) decrease of 27.88%. Conclusion:The present study showed a negative correlation between %MetHb and MDA levels in human erythrocytes incubated with ASA over time, suggesting that the erythrocytes redox status did not specifically induce membrane lipid peroxidation.

KEYWORDS

• Acetylsalicylic Acid
• Erythrocyte
• Malondialdehyde
• Methaemoglobin

Showing references and in-text citations exactly as published.

• DOI10.5530/fra.2024.2.12Copy

RELATED ARTICLES

Matched on section and article type within this issue of Free Rad. Antiox..

• Article · VOLUME 14, ISSUE 2Wound-Healing Activity of Herbal Ointment Containing the Root Extracts of Astragalus propinquus in Sprague Dawley RatsVaratharajan Rajavel, Fazlina Mustaffa, Foo Yau Rou, Lim Jia Qi, Catherine Khor Khai Lin, Mari Jothi, Karthikeyan Jayabalan · pp. 104–110Original ArticleSame type: Article
• Article · VOLUME 14, ISSUE 2Evaluation of Free Radical Scavenging Efficacy of Siddha Formulation Pirandai uppu Using in vitro Antioxidant AssayKanimozhi Selvakumaran, Sathish Adithya Rajadhinakaran, Nandhini Elangovan, Subhashree H · pp. 111–118Original ArticleSame type: Article
• Article · VOLUME 14, ISSUE 2Evaluation of the Antioxidative and Qualitative Properties of the Tinospora cordifoliaArthi Boro, Krishnaswamy Sujatha, Jothi Dheivasikamani Abidharini, Pragya Pallavi, Jeyabal Philomenathan Antony Prabhu, Arumugam Vijaya Anand · pp. 126–130Original ArticleSame type: Article
• Article · VOLUME 14, ISSUE 22,3,4-Trihydroxybenzoic Acid, an Antioxidant Plant Metabolite Inhibits Cancer Cell Growth in vitro: Potential Role in Cancer PreventionMina Akter, Md. Imtiazul Kabir, Nasiruddin Nalban, Srinivasan Marimuthu, Ranjini Sankaranarayanan, G. Jayarama Bhat · pp. 86–96Original ArticleSame type: Article