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    Free Radicals and AntioxidantsNOW IN
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    Membrane stability and methaemoglobin content of human erythrocytes incubated in aqueous leaf extract of Nicotiana tabacum product

    Chikezie P.C.1★, Akuwudike A. R.1, Chikezie C. M.1★ Corresponding author

    1. 1Department of Biochemistry, Imo State University, PMB 2000, Owerri, Nigeria.

    CORRESPONDENCE

    Chikezie P.C.

    Department of Biochemistry, Imo State University, PMB 2000, Owerri, Nigeria.

    p_chikezie@yahoo.com

    Received: 12-10-2012; Revised: 01-12-2012; Accepted: 17-12-2012.

    Volume 2, Issue 4 · pp. 56–61 · PUBLISHED · DOI: 10.5530/ax.2012.4.10

    View on Free Rad. Antiox. original site ↗

    ABSTRACT

    The present study seeks to ascertain membrane stability and methaemoglobin content (%MetHb) of human erythrocytes suspended in aqueous leaf extract of N. tabacum. Erythrocyte membrane stability (%EMS) and %MetHb were measured by spectrophotometric method. Erythrocytes incubated in the five experimental concentrations of N. tabacum exhibited higher tendency to haemolyze in phosphate buffered saline solution [PBS] > 0.54 g/100 mL, whereas erythrocytes incubated in aqueous extracts of [N. tabaccum] = 0.4 and 0.8 mg/100 mL showed relatively lower level of haemolysis in [PBS] Membrane stability Methaemoglobin N. tabacum Erythrocytes

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      P.C., C., R., A. A., & M., C. C. (). Membrane stability and methaemoglobin content of human erythrocytes incubated in aqueous leaf extract of Nicotiana tabacum product. Free Radicals and Antioxidants, 2(4), 56–61. https://doi.org/10.5530/ax.2012.4.10