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    Effect of the pretreatment with acerola (Malpighia emarginata DC.) juice on ethanol-induced oxidative stress in mice e Hepatoprotective potential of acerola juice

    Neuza Félix Gomes Rochette1, Erika Freitas Mota2, Diana Célia Sousa Nunes-Pinheiro3, Camila Freitas Bezerra1, Maria Liduína Maia de Oliveira3, Ana Claudia Marinho da Silva4, Maria Raquel Alcântara de Miranda1, Dirce Fernandes de Melo1★★ Corresponding author

    1. 1Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, 60455-970 Fortaleza, Ceará, Brazil.
    2. 2Departamento de Biologia, Universidade Federal do Ceará, 60440-554 Fortaleza, Ceará, Brazil.
    3. 3Faculdade de Veterinária, Universidade Estadual do Ceará, 60740-903 Fortaleza, Ceará, Brazil.
    4. 4Faculdade Católica do Ceará, Av. Duque de Caxias, 60035-110 Fortaleza, Ceará, Brazil.

    CORRESPONDENCE

    Dirce Fernandes de Melo

    Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, 60455-970 Fortaleza, Ceará, Brazil.

    dirfmelo@gmail.com

    Received: 25-05-2013; Revised: 11-10-2013; Accepted: 12-11-2013.

    Volume 3, Issue 2S · pp. s16–s21 · PUBLISHED · DOI: 10.1016/j.fra.2013.10.004

    View on Free Rad. Antiox. original site ↗

    ABSTRACT

    Background and objectives: Acerola (Malpighia emarginata DC.) is a tropical fruit known for its nutritional and functional properties due to its great contents vitamin C, carotenoids and anthocyanins. The anti- oxidant potential from plants extracts associated to hepatoprotective activity has been widely studied. However, the effect of antioxidants in natural fruit juice has not been fully searched. The aim of this work was to investigate the antioxidant properties of acerola juice and its hepatoprotective potential against acute ethanol-induced stress. Materials and methods:Ripe acerola were collected, processed into juice and initially analyzed to anti- oxidant properties in vitro. Afterwards, in vivo hepatoprotective activity was evaluated in mice. The animals received the juice by gavage as pretreatment for 15 consecutive days and then, were submitted to ethanol-induced stress in single dose (5 g/kg). The activities of serum enzymes as well as lipid per- oxidation degree were evaluated. The activities of serum marker enzymes for liver damage as well as lipid peroxidation degree were evaluated. Results: Acerola juice presents great vitamin C (1799.5 mg/100 g FW) and total phenolic (188.4 mg GAE/ 100 g FW), anthocyanins (9.2 mg/100 g FW), flavonols (7.8 mg/100 g FW) contents and high activity of superoxide dismutase (1053.6 UA/g DM) with a total antioxidant activity of 137.5 mmol Trolox/g FW. The juice treatment inhibited lipid peroxidation and reduced the activities of aminotransferases (p �0.05), in mice liver. Conclusion: These results indicate that acerola juice is able to prevent the hepatic damage induced by ethanol, probably as a result of an enhancement of the antioxidant status in the animals.

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      Rochette, N. F. G., Mota, E. F., Nunes-Pinheiro, D. C. S., Bezerra, C. F., Oliveira, M. L. M. D., Silva, A. C. M. D., Miranda, M. R. A. D., & Melo, D. F. D. (). Effect of the pretreatment with acerola (Malpighia emarginata DC.) juice on ethanol-induced oxidative stress in mice e Hepatoprotective potential of acerola juice. Free Radicals and Antioxidants, 3(2S), s16–s21. https://doi.org/10.1016/j.fra.2013.10.004