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    Free Radicals and AntioxidantsNOW IN
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    Protective effect of Ficus bengalensis L. extract against H2 O2 induced DNA damage and repair in neuroblastoma cells

    V. Ramakrishna1,2★, K. Preeti Guptab1, Oruganti H. Settyb1, Anand K. Kondapia2★ Corresponding author

    1. 1Departments of Biotechnology, School of Life Sciences, University of Hyderabad, Hyderabad –500 046, INDIA.
    2. 2Departments of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad –500 046, INDIA.

    CORRESPONDENCE

    V. Ramakrishna

    Departments of Biotechnology, School of Life Sciences, University of Hyderabad, Hyderabad –500 046, INDIA.

    vrkrishna70@gmail.com

    Volume 4, Issue 1 · pp. 3–7 · PUBLISHED · DOI: 10.5530/fra.2014.1.2

    View on Free Rad. Antiox. original site ↗

    ABSTRACT

    Aim & background:Neuronal cells, because of their high rate of oxidative metabolism and low levels of antioxidants, are quite susceptible to oxidative DNA damage. Ficus bengalensis L. (Banyan tree) is a large tree and possessing medicinal properties. The study was designed to investigate the protective effect of Ficus bengalensis (FB) extracts against H2 O2 induced DNA damage and repair in human neuroblastoma cells (SK N SH).Methods:Aqueous and methanol extracts of Ficus bengalensis were prepared and cell viability conferred by these plant extracts was evaluated by MTT assay. DNA damage and protection by FB extracts was measured by comet assay using H2 O2 as DNA damaging agent. Results: Cell viability markedly decreased with increased DNA damage after a 24 h exposure to H2 O2 . However, prior incubation with FB extract (0.1 – 1.0 mg/ml) for 24 h, and then exposed to H2 O2 , cellular toxicity was significantly attenuated in a dosedependent manner. Oxidative DNA damage in FB treated cells showed a significant reduction in intensity of DNA damage in terms of comet tail length and also brought to control level. Conclusion: The study indicated Ficus bengalensis extract showing potential neuroprotective activity against oxidative environment generated by H2 O2 treatment.

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      Ramakrishna, V., Guptab, K. P., Settyb, O. H., & Kondapia, A. K. (). Protective effect of Ficus bengalensis L. extract against H2 O2 induced DNA damage and repair in neuroblastoma cells. Free Radicals and Antioxidants, 4(1), 3–7. https://doi.org/10.5530/fra.2014.1.2