Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Free Radicals and AntioxidantsNOW IN
    Article

    In vitro antioxidant and antimicrobial activities of propolis from Kashmir Himalaya region

    Adil Farooq Wali1, Ahlam Mushtaq2, Muneeb U Rehman3, Seema Akbar4, Mubashir Hussain Masoodi1★★ Corresponding author

    1. 1Department of Pharmaceutical Sciences, University of Kashmir, Srinagar, Jammu and Kashmir, 190006, INDIA.
    2. 2Department of Biochemistry, University of Kashmir, Srinagar, Jammu and Kashmir, 190006, INDIA.
    3. 3Department of Biochemistry, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e- Kashmir University of Agricultural Sciences and Technology Kashmir, Jammu and Kashmir 190006, INDIA.
    4. 4Central Council for Research in Unani Medicine, University of Kashmir, Srinagar, Jammu and Kashmir 190006, INDIA.

    CORRESPONDENCE

    Mubashir Hussain Masoodi

    Department of Pharmaceutical Sciences, University of Kashmir, Srinagar, Jammu and Kashmir, 190006, INDIA.

    mubashir@kashmiruniversity.ac.in

    Volume 6, Issue 1 · pp. 51–57 · PUBLISHED · DOI: 10.5530/fra.2016.1.6

    View on Free Rad. Antiox. original site ↗

    ABSTRACT

    Introduction: Propolis from Kashmir has not been explored as far as its phytochemistry and pharmacological activities are concerned. This piece of work is the first approach to explore it for various activities. Methods: Total phenolics, flavonoids contents and in vitro antioxidant assay such DPPH, H2 O2 scavenging assay, ferrous metal ion chelating activity and total reduction capability assay were performed. Investigation for antimicrobial activity of different extracts (Ethanolic, hydro- ethanolic and aqueous) was also carried out. Results:The results showed highest total phenolics and flavonoids content in ethanolic extract (260 ± 10.00 mg GAE/g and 105 ± 5.00 mg QE/g respectively). All the extracts showed free radical scavenging potential but ethanolic extract exhibited significant scavenging potential having an IC50 value of 65.49 ± 7.01 µg/mL for DPPH, 74.94 ± 5.51 µg/mL for ferrous metal ion chelating activity and 109.93 ± 3.24 µg/mL for H2 O2 scavenging assay. In total reduction capability assay ethanolic extract showed highest reducing power. In addition, ethanolic extract showed greater antibacterial activity against Staphylococcus aureus having zone of inhibition diameter 25.63 ± 0.63 mm. Conclusion:The presence of flavonoids in extracts maximum in ethanolic extract which could be responsible for antioxidant, antibacterial activities and can be used in the management of the human pathosis originated due to oxidative stress and in the treatment of microbial infections.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Wali, A. F., Mushtaq, A., Rehman, M. U., Akbar, S., & Masoodi, M. H. (). In vitro antioxidant and antimicrobial activities of propolis from Kashmir Himalaya region. Free Radicals and Antioxidants, 6(1), 51–57. https://doi.org/10.5530/fra.2016.1.6