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    Journal of Pharmacology and PharmacotherapeuticsNOW IN
    Article

    Antiproliferative property of n-hexane and chloroform extracts of Anisomeles malabarica (L). R. Br. in HPV16-positive human cervical cancer cells

    Christo Paul Preethy2★, Ramamoorthy Padmapriya2, Vaiyapuri Subbarayan Periasamy2,3, Anvarbatcha Riyasdeen2, Suresh Srinag4, Hanumanthappa Krishnamurthy4, Ali Abdullah Alshatwi3, Mohammad Abdulkader Akbarsha3,5★ Corresponding author

    1. 1Department of Animal Science, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India.
    2. 2Department of Food Sciences and Nutrition, College of Food Sciences and Agriculture, King Saud University, Riyadh, Kingdom of Saudi Arabia.
    3. 3National Center for Biological Sciences, Tata Institute of Fundamental Research, Bangalore.
    4. 4Mahatma Gandhi Doerenkamp Center for Alternatives to Use of Animals in Life Science Education, Bharathidasan University, Tiruchirappalli,Tamil Nadu, India Research Paper.

    CORRESPONDENCE

    Christo Paul Preethy

    Department of Food Sciences and Nutrition, College of Food Sciences and Agriculture, King Saud University, Riyadh, Kingdom of Saudi Arabia.

    Volume 3, Issue 1 · pp. 26–34 · PUBLISHED 2012 · DOI: 10.4103/0976-500X.92500

    View on J Pharmacol. Pharmacother. original site ↗

    ABSTRACT

    Objectives: To find the efficacy of serial extracts of Anisomeles malabarica in inhibiting proliferation of and inducing apoptosis in human cervical cancer cells, SiHa and ME 180, that are HPV 16-positive. Materials and Methods: The whole plant was extracted in n-hexane, chloroform, ethyl acetate, n-butanol, methanol, and water. The cells were treated with the extracts at increasing concentrations to find the IC50, adopting MTT ([3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide]) assay. Acridine orange (AO) and ethidium bromide (EB) and Hoechst 33258 staining were adopted to assess the mode of cell death, Annexin V-Cy3 staining to evaluate one of the early apoptotic features, JC-1 staining to assess the mitochondrial membrane depolarization, comet assay for DNA fragmentation, and cell cycle analysis for the distribution of cells after treatment. Results: n-Hexane and chloroform extracts were cytotoxic to the cervical cancer cells in dose- and duration-dependent manner. The cells that responded to the treatments revealed typical apoptotic features. Early features of apoptosis, phosphatidyl serine translocation and loss of mitochondrial trans-membrane potential, were observed in the treated cells, and comet assay revealed DNA damage. In the FACS analysis, the cells accumulated in the sub-G0/G1 phase of the cell cycle, except in n-hexane- and chloroform extract–treated SiHa cells at 24 h, which showed arrest in S- and G2/M phases. Conclusions: n-Hexane and chloroform extracts of A. malabarica inhibit proliferation of and induce death in HPV16-positive cervical cancer cells, mostly by apoptosis and to some extent by necrosis.

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      Preethy, C. P., Padmapriya, R., Periasamy, V. S., Riyasdeen, A., Srinag, S., Krishnamurthy, H., Alshatwi, A. A., & Akbarsha, M. A. (2012). Antiproliferative property of n-hexane and chloroform extracts of Anisomeles malabarica (L). R. Br. in HPV16-positive human cervical cancer cells. Journal of Pharmacology and Pharmacotherapeutics, 3(1), 26–34. https://doi.org/10.4103/0976-500X.92500