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    Article

    Effect of ultrasound on the isoflavonoid production in Genista tinctoria L. suspension cultures

    Lenka Tůmová1★, Jiří Tůma2, Helena Hendrychová1★ Corresponding author

    1. 1Department of Pharmacognosy, Faculty of Pharmacy in Hradec Králové, Charles University Prague, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic.
    2. 2Department of Biology, University of Hradec Králové, Faculty of Natural Sciences, Rokitanského 62 500 03 Hradec Králové, Czech Republic.

    CORRESPONDENCE

    Lenka Tůmová

    Department of Pharmacognosy, Faculty of Pharmacy in Hradec Králové, Charles University Prague, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic.

    tumova@faf.cuni.cz

    Received: 11-12-2012; Revised: 29-04-2013.

    Volume 10, Issue 38s · pp. 425–429 · PUBLISHED 28 May 2014 · DOI: 10.4103/0973-1296.133297

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Application of ultrasound (US) to biotechnology is relatively new but several processes that take place in the presence of cells or enzymes are activated by ultrasonic waves. Genista tinctoria L. (Fabaceae) is rich on various kind of flavonoids, including isoflavones with valuable estrogenic activity. Objective: This study verified use of low‑energy US elicitor to enhance secondary metabolite production in plant cell cultures. Materials and Methods: Suspension cultures of G. tinctoria cells was exposed to low‑power US (with fixed frequency 35 kHz and power level 0.1 mW/cm3) for period 1‑5 min. Results: The US exposure significantly stimulated genistin content (0.8 mg/g DW) after 3 min of US treatment (sampled after 72 h). The highest daidzein level (1.4 mg/g DW) was reached after US irradiation for 5 min and 168 h sampling. Conclusion: The achieved results suggest that US can act as a potent abiotic elicitor to induce the defense responses of plant cells and to stimulate secondary metabolite production in plant cell cultures.

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      Tůmová, L., Tůma, J., & Hendrychová, H. (2014). Effect of ultrasound on the isoflavonoid production in Genista tinctoria L. suspension cultures. Pharmacognosy Magazine, 10(38s), 425–429. https://doi.org/10.4103/0973-1296.133297