Simultaneous determination of esculetin, quercetin‑3‑O‑β‑D‑glucuronide, quercetin‑3‑O‑β ‑D-glucuronopyranside methyl ester and quercetin in effective part of Polygonum Perfoliatum L. using high performace liquid chromatography
Dongsheng Fan2, Yang Zhao2,3, Xin Zhou2,3★, Xiaojian Gong2,3, Chao Zhao2,3★ Corresponding author
- 1The Research Center for Quality Control of Natural Medicine, China.
- 2Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Enviroment, Guizhou Normal University, Guiyang, China.
CORRESPONDENCE
Xin Zhou
Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Enviroment, Guizhou Normal University, Guiyang, China.
Received: 15-10-2013; Revised: 20-11-2013.
Volume 10, Issue 39 · pp. 359–366 · PUBLISHED 24 July 2014 · DOI: 10.4103/0973-1296.137379
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ABSTRACT
Objective: In the present study, a high performance liquid chromatography (HPLC) coupled with photodiode array detection was developed for simultaneous quantitation of esculetin, quercetin‑3‑O‑b‑D‑glucuronide, quercetin‑3‑O‑b‑D‑ glucuronopyranoside methyl ester and quercetin in Polygonum perfoliatum L. Materials and Methods: The chromatographic separations were performed on a reversed‑phase C18 column using a mobile phase composed of acetonitrile –0.5% aqueous acetic acid with gradient elution. The calibration curves for the analytes demonstrated good linearities within the investigated ranges. The satisfactory intra‑ and inter‑day precision, repeatability and stability of the developed analytical method were shown in the method validation procedure. The recoveries of the established method ranged from 95.76 to 102.10% for all the analytes. Results: This proposed method was successfully applied for simultaneous quantification of the four compounds in effective part of Polygonum perfoliatum L. from different regions. Hierarchical clustering analysis (HCA) and principal components analysis (PCA) were performed to characterize and classify the samples based on the contents of the four compounds in Polygonum perfoliatum L. Conclusion: The established HPLC method combined with chemometric approaches was proven to be useful and efficient for quality control of Polygonum perfoliatum L.
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Fan, D., Zhao, Y., Zhou, X., Gong, X., & Zhao, C. (2014). Simultaneous determination of esculetin, quercetin‑3‑O‑β‑D‑glucuronide, quercetin‑3‑O‑β ‑D-glucuronopyranside methyl ester and quercetin in effective part of Polygonum Perfoliatum L. using high performace liquid chromatography. Pharmacognosy Magazine, 10(39), 359–366. https://doi.org/10.4103/0973-1296.137379