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    Article

    Microwave‑assisted extraction of polysaccharides from Yupingfeng powder and their antioxidant activity

    Dan Wang1, Bi‑Bo Zhang1, Xiao‑Xia Qu1, Feng Gao1★, Min‑Yong Yuan2★ Corresponding author

    1. 1Department of Medicinal Plants, Agronomy College, Sichuan Agricultural University, Wenjiang Region, Chengdu 611130, China.
    2. 2Department of Pharmacy, The First Affiliated Hospital of Chengdu Medical College, Xindu Region, Chengdu 610500, P. R, China.

    CORRESPONDENCE

    Feng Gao

    Department of Medicinal Plants, Agronomy College, Sichuan Agricultural University, Wenjiang Region, Chengdu 611130, China.

    gaofeng@sicau.edu.cn

    Received: 24-10-2014; Revised: 27-12-2014.

    Volume 11, Issue 43 · pp. 546–554 · PUBLISHED 10 July 2015 · DOI: 10.4103/0973-1296.160468

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Microwave‑assisted reflux extraction of polysaccharides YPF-P from the famous Chinese traditional drug, Yupingfeng powder, optimization of extracting conditions and evaluation of their antioxidant activity were conducted in this study. Results: Single factor effect trends were achieved through yields and contends of YPF‑P obtained from different extracting conditions. Then through a three‑level, four‑variable Box‑Behnken design of response surface methodology adopting yield as response, the optimal conditions were determined as follows: Material/solvent ratio 1:23.37, microwave power 560 W, Extraction temperature 64°C, and extraction time 9.62 min. Under the optimal conditions, the YPF‑P extraction yield was 3.23%, and its content was detected as 38.52%. In antioxidant assays, the YPF‑P was tested to possess 1,1‑diphenyl‑2‑picrylhydrazyl (DPPH) radical scavenging activities with an IC50 value of 0.262 mg/ml. In addition, YPF‑P was also proved to have relatively low ferric reducing antioxidant power (FRAP), compared to Vc, through FRAP assay. Conclusion: In the microwave‑assisted reflux extraction research, good YPF‑P yield was achieved from materials with relatively low YPF‑P content. And for the first time, both DPPH and FRAP assays were conducted on YPF‑P, which proved that the antioxidant activity of YPF‑P contributed to the functions of this medicine.

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      Wang, D., Zhang, B., Qu, X., Gao, F., & Yuan, M. (2015). Microwave‑assisted extraction of polysaccharides from Yupingfeng powder and their antioxidant activity. Pharmacognosy Magazine, 11(43), 546–554. https://doi.org/10.4103/0973-1296.160468