Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy MagazineNOW IN
    Article

    Reversed‑phase‑liquid chromatography method for separation and quantification of gallic acid from hydroalcoholic extracts of Qualea grandiflora and Qualea parviflora

    Mariana L. de Mesquita2,3,4, Waleska F. Leão3,4, Magda R. A. Ferreira3, José E. de Paula5, Laila S. Espindola2, Luiz A. L. Soares3,4★★ Corresponding author

    1. 1Laboratory of Pharmacognosy, University of Brasília – UnB, Brasília-DF, Brazil.
    2. 2Post Graduate Programme in Therapeutic Innovation, Federal University of Pernambuco – UFPE, Recife-PE, Brazil.
    3. 3Pharmacognosy Laboratory, Federal University of Pernambuco – UFPE, Recife-PE, Brazil.
    4. 4Plant Anatomy Laboratory, University of Brasília – UnB; Brasília-DF, Brazil.

    CORRESPONDENCE

    Luiz A. L. Soares

    Pharmacognosy Laboratory, Federal University of Pernambuco – UFPE, Recife-PE, Brazil.

    lals@pq.cnpq.br

    Received: 26-06-2013; Revised: 17-08-2013.

    Volume 11, Issue 44s1 · pp. 316–321 · PUBLISHED 24 September 2015 · DOI: 10.4103/0973-1296.166062

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Qualea parviflora and Qualea grandiflora (Vochysiaceae), commonly known in Brazil as “pau‑terra” and “pau‑terrinha,” respectively, have been widely used in the treatment of ulcer and gastritis. These therapeutic effects are attributed to various compounds present in the plants, including phenolic compounds such as gallic acid, due to their important antioxidant activity. Objective: The aim of the present study was to validate a high performance liquid chromatography with diode array detection (HPLC‑DAD) method for the quantitative determination of gallic acid in the stem bark of Q. parviflora and Q. grandiflora hydroalcoholic extracts. Materials and Methods: The chromatography analysis was successfully achieved on a Dionex column, Acclaim® 120 (250 mm × 4.60 mm, 5 µm) with a gradient elution of water and methanol at a flow rate of 0.8 mL/min and ultraviolet detection at 280 nm. Results: The validation data, including linearity, precision, specificity, accuracy and robustness of this method demonstrated good reliability and sensitivity. Conclusion: The method is able to quantify gallic acid in the stem bark of both species. What is more, the chromatographic peaks showed good resolution and there are also the advantages of easy sample preparation and a short time between each injection.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Mesquita, M. L. D., Leão, W. F., Ferreira, M. R. A., Paula, J. E. D., Espindola, L. S., & Soares, L. A. L. (2015). Reversed‑phase‑liquid chromatography method for separation and quantification of gallic acid from hydroalcoholic extracts of Qualea grandiflora and Qualea parviflora. Pharmacognosy Magazine, 11(44s1), 316–321. https://doi.org/10.4103/0973-1296.166062