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    Toxicological Evaluation of Emblica officinalis Fruit Extract and its Anti‑inflammatory and Free Radical Scavenging Properties

    Sushil Kumar Middha1, Arvind Kumar Goyal2, Prakash Lokesh3, Varsha Yardi6, Lavanya Mojamdar1, Deepthi Sudhir Keni4, Dinesh Babu5, Talambedu Usha6★★ Corresponding author

    1. 1Departments of Biotechnology, College for Women, Malleswarm.
    2. 2Department of Biotechnology, Bamboo Technology, Bodoland University, Kokrajhar, BTAD, Assam.
    3. 3Department of Biochemistry, CPGS, Jain College.
    4. 4Department of Biotechnology, Sapthagiri College of Engineering, Bengaluru, Karnataka.
    5. 5Heymans Institute of Pharmacology, Ghent University, Belgium.
    6. 6Biochemistry, Maharani Lakshmi Ammanni College for Women, Malleswarm.

    CORRESPONDENCE

    Talambedu Usha

    Biochemistry, Maharani Lakshmi Ammanni College for Women, Malleswarm.

    ushatalambedu@gmail.com

    Volume 11, Issue 44s2 · pp. 427–433 · PUBLISHED 2015 · DOI: 10.4103/0973-1296.168982

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Emblica officinalis (Euphorbiaceae), popularly known as Indian gooseberry or “Amla” in India, is used in Ayurveda as “rejuvenating herb” since ancient times. Objective: This study was carried out to estimate toxicity, anti‑inflammatory, and antioxidative activities of the methanolic extract of Emblica officinalis fruit (MEO) in an animal model. Materials and Methods: Antioxidative property of MEO was assessed by in vitro assays such as phosphomolybdenum assay (total antioxidant capacity), free radical scavenging assays 1,1‑diphenyl‑2‑picrylhydrazyl and 2,2’‑azino‑bis and 3‑ethylbenzthiazoline‑6‑sulphonic acid (DPPH and ABTS method) and lipid peroxidation assay (LPO). The anti‑inflammatory property was evaluated by carrageenan‑induced acute inflammation in rats by measuring rat paw volume at different time intervals and toxicological analysis using mice. Results and Discussion: High performance liquid chromatography studies revealed the presence of gallic acid (2.10%), mucic acid (4.90%), ellagic acid (2.10%), quercetin (28.00%), rutin (3.89%), and β‑glucogallin (1.46%). MEO showed highest antioxidant activities by using DPPH (17.33–89.00%), ABTS (23.03–94.16%), nitric oxide scavenging activity (12.94–70.16%), LPO (56.54%), and phosphomolybdenum assay (142 ± 6.09 µg/ml). The LD50 was found to be approximately 1125 mg/kg (p.o). High dose of MEO showed significant reduction (72.71%) in the inflammation after 4 h of treatment, which was comparable to diclofenac (10 mg/kg) (61.57%) treated group. Significant reduction (P < 0.05) in the inflammatory cytokine (interleukin‑1β and tumor necrosis factor‑α) markers were also observed (57.25% and 35.41%, respectively) in serum of MEO treated animals as compared to control. Conclusion: Taken together, phenolic compounds of MEO may serve as a potential herbal drug for amelioration of acute inflammation due to their modulatory action on free radicals.

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      Middha, S. K., Goyal, A. K., Lokesh, P., Yardi, V., Mojamdar, L., Keni, D. S., Babu, D., & Usha, T. (2015). Toxicological Evaluation of Emblica officinalis Fruit Extract and its Anti‑inflammatory and Free Radical Scavenging Properties. Pharmacognosy Magazine, 11(44s2), 427–433. https://doi.org/10.4103/0973-1296.168982