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    7‑hydroxycalamenene Effects on Secreted Aspartic Proteases Activity and Biofilm Formation of Candida spp.

    Mariana M. B. Azevedo1★, Catia A. Almeida1, Francisco C. M. Chaves2, Igor A. Rodrigues3, Humberto R. Bizzo1★ Corresponding author

    1. 1Celuta S. Alviano, Daniela S. Alviano Department of General Microbiology, Institute of Microbiology Paulo de Góes, Federal Universityof Rio de Janeiro, CCS, Fundão Island, Rio de Janeiro, RJ, Brazil.
    2. 2EMBRAPA Western Amazon, Rodovia AM 10, Manaus, AM, Brazil.
    3. 3Department of Natural Products and Food, Federal University of Rio de Janeiro, CCS, Fundão Island, Rio de Janeiro, RJ, Brazil.
    4. 4EMBRAPA Food Technology, Avenue of Americas, Rio de Janeiro, RJ, Brazil.

    CORRESPONDENCE

    Mariana M. B. Azevedo

    Celuta S. Alviano, Daniela S. Alviano Department of General Microbiology, Institute of Microbiology Paulo de Góes, Federal Universityof Rio de Janeiro, CCS, Fundão Island, Rio de Janeiro, RJ, Brazil.

    marimbarros@gmail.com

    Volume 12, Issue 45 · pp. 36–40 · PUBLISHED 2016 · DOI: 10.4103/0973-1296.176022

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: The 7‑hydroxycalamenenene‑rich essential oil (EO) obtained from the leaves of Croton cajucara (red morphotype) have been described as active against bacteria, protozoa, and fungi species. In this work, we aimed to evaluate the effectiveness of 7‑hydroxycalamenenene against Candida albicans and nonalbicans species. Materials and Methods: C. cajucara EO was obtained by hydrodistillation and its major compound, 7‑hydroxycalamenene, was purified using preparative column chromatography. The anti‑candidal activity was investigated by minimum inhibitory concentration (MIC) and secreted aspartic proteases (SAP) and biofilm inhibition assays. Results: 7‑hydroxycalamenene (98% purity) displayed anti‑candidal activity against all Candida species tested. Higher activity was observed against Candida dubliniensis, Candida parapsilosis and Candida albicans, showing MIC values ranging from 39.06 µg/ml to 78.12 µg/ml. The purified 7‑hydroxycalamenene was able to inhibit 58% of C. albicans ATCC 36801 SAP activity at MIC concentration (pH 7.0). However, 7‑hydroxycalamenene demonstrated poor inhibitory activity on C. albicans ATCC 10231 biofilm formation even at the highest concentration tested (2500 µg/ml). Conclusion: The bioactive potential of 7‑hydroxycalamenene against planktonic Candida spp. further supports its use for the development of antimicrobials with anti‑candidal activity.

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      Azevedo, M. M. B., Almeida, C. A., Chaves, F. C. M., Rodrigues, I. A., & Bizzo, H. R. (2016). 7‑hydroxycalamenene Effects on Secreted Aspartic Proteases Activity and Biofilm Formation of Candida spp.. Pharmacognosy Magazine, 12(45), 36–40. https://doi.org/10.4103/0973-1296.176022