The Attenuation of Scutellariae radix Extract on Oxidative Stress for Colon Injury in Lipopolysaccharide‑induced RAW264.7 Cell and 2,4,6‑trinitrobenzene Sulfonic Acid‑induced Ulcerative Colitis Rats
Yu Jin1, Jun Yang1, Lianjie Lin1, Yan Lin1, Changqing Zheng1★★ Corresponding author
- 1Department of Gastroenterology, Shengjing Hospital of China Medical University, Tiexi District, Shenyang 110021, Liaoning, China ORIGINAL ARTICLE.
CORRESPONDENCE
Changqing Zheng
Department of Gastroenterology, Shengjing Hospital of China Medical University, Tiexi District, Shenyang 110021, Liaoning, China ORIGINAL ARTICLE.
Received: 13-03-2015; Revised: 02-04-2015.
Volume 12, Issue 46 · pp. 153–159 · PUBLISHED 2 March 2016 · DOI: 10.4103/0973-1296.177913
View on Pharmacogn. Mag. original site ↗
ABSTRACT
Background: Oxidative stress (OS) has been regarded as one of the major pathogeneses of ulcerative colitis (UC) through damaging colon. It has been shown that Scutellariae radix (SR) extract has a beneficial effect for the prevention and treatment of UC. Objective: The aim of this study was to investigate whether SR had a potential capacity on oxidant damage for colon injury both in vivo and in vitro. Materials and Methods: The 2,4,6‑trinitrobenzene sulfonic acid (TNBS) was used to induce UC rats model while 1 μg/ml lipopolysaccharide (LPS) was for RAW264.7 cell damage. Disease activity index (DAI) was determined to response the severity of colitis. The myeloperoxidase (MPO) activity in rat colon was also estimated. The 2,2’‑azino‑bis‑3‑ethylbenzthiazoline‑6‑sulfonic acid assay was performed to evaluate the total antioxidant capacity of SR. Furthermore, the activity of glutathione peroxidase (GSH‑PX), catalase (CAT), superoxide dismutase (SOD), and lipid peroxidation malondialdehyde (MDA) in cell supernatant and rat serum were detected by appropriate kits. In addition, an immunohistochemical assay was applied to examine transforming growth factor beta 1 (TGF‑β1) protein expression in colon tissue. Results: The treatment with SR could significantly increase the activity of GSH‑PX, CAT, and SOD associated with OS in LPS‑induced RAW264.7 cell damage and TNBS‑induced UC rats. However, the level of MDA was markedly reduced both in vitro and in vivo. Furthermore, SR significantly decreased DAI and reversed the increased MPO activity. Thus, SR could decrease the severity of acute TNBS‑induced colitis in rats. Immunohistochemical assay showed that SR significantly downregulated TGF‑β1 protein expression in colon tissue. Conclusion: Our data provided evidence to support this fact that SR attenuated OS in LPS‑induced RAW264.7 cell and also in TNBS‑induced UC rats. Thus, SR may be an interesting candidate drug for the management of UC.
KEYWORDS
REFERENCES
As publishedShowing references and in-text citations exactly as published.
Cite this article
SELECT FORMAT
Jin, Y., Yang, J., Lin, L., Lin, Y., & Zheng, C. (2016). The Attenuation of Scutellariae radix Extract on Oxidative Stress for Colon Injury in Lipopolysaccharide‑induced RAW264.7 Cell and 2,4,6‑trinitrobenzene Sulfonic Acid‑induced Ulcerative Colitis Rats. Pharmacognosy Magazine, 12(46), 153–159. https://doi.org/10.4103/0973-1296.177913