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    In vitro Cytotoxicity and Anti‑herpes Simplex Virus Type 1 Activity of Hydroethanolic Extract, Fractions, and Isolated Compounds from Stem Bark of Schinus terebinthifolius Raddi

    Samara Requena Nocchi2, Gislaine Franco de Moura‑Costa2, Claudio Roberto Novello3, Juliana Rodrigues2, Renata Longhini4, João Carlos Palazzo de Mello2,4, Benedito Prado Dias Filho2,5, Celso Vataru Nakamura2,5, Tânia Ueda‑Nakamura2,5★★ Corresponding author

    1. 1Post-Graduate Program in Pharmaceutical Sciences, State University of Maringá.
    2. 2Coordination of Chemical Engineering, Federal Technical University of Paraná, Francisco Beltrão Campus, Santa Barbara Line s/n, BR-85601- 970, Francisco Beltrão, Paraná, Brazil.
    3. 3Department of Pharmacy, State University of Maringá.
    4. 4Department of Health Basic Sciences, State University of Maringá, Av. Colombo 5790, BR-87020-900, Maringá, Paraná, Brazil Pharmacogn. Mag. A multifaceted peer reviewed journal in the field of Pharmacognosy and Natural Products.

    CORRESPONDENCE

    Tânia Ueda‑Nakamura

    Coordination of Chemical Engineering, Federal Technical University of Paraná, Francisco Beltrão Campus, Santa Barbara Line s/n, BR-85601- 970, Francisco Beltrão, Paraná, Brazil.

    tunakamura@uem.br

    Received: 26-05-2015; Revised: 04-08-2015.

    Volume 12, Issue 46 · pp. 160–164 · PUBLISHED 2 March 2016 · DOI: 10.4103/0973-1296.177903

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Herpes simplex virus type 1 (HSV‑1) is associated with orofacial infections and is transmitted by direct contact with infected secretions. Several efforts have been expended in the search for drugs to the treatment for herpes. Schinus terebinthifolius is used in several illnesses and among them, for the topical treatment of skin wounds, especially wounds of mucous membranes, whether infected or not. Objective: To evaluate the cytotoxicity and anti‑HSV‑1 activity of the crude hydroethanolic extract (CHE) from the stem bark of S. terebinthifolius, as well as its fractions and isolated compounds. Materials and Methods: The CHE was subjected to bioguided fractionation. The anti‑HSV‑1 activity and the cytotoxicity of the CHE, its fractions, and isolated compounds were evaluated in vitro by SRB method. A preliminar investigation of the action of CHE in the virus–host interaction was conducted by the same assay. Results: CHE presented flavan‑3‑ols and showed anti‑HSV‑1 activity, better than its fractions and isolated compounds. The class of substances found in CHE can bind to proteins to form unstable complexes and enveloped viruses, as HSV‑1 may be vulnerable to this action. Our results suggest that the CHE interfered with virion envelope structures, masking viral receptors that are necessary for adsorption or entry into host cells. Conclusion: The plant investigated exhibited potential for future development treatment against HSV‑1, but further tests are necessary, especially to elucidate the mechanism of action of CHE, as well as preclinical and clinical studies to confirm its safety and efficacy.

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      Nocchi, S. R., Moura‑Costa, G. F. D., Novello, C. R., Rodrigues, J., Longhini, R., Mello, J. C. P. D., Filho, B. P. D., Nakamura, C. V., & Ueda‑Nakamura, T. (2016). In vitro Cytotoxicity and Anti‑herpes Simplex Virus Type 1 Activity of Hydroethanolic Extract, Fractions, and Isolated Compounds from Stem Bark of Schinus terebinthifolius Raddi. Pharmacognosy Magazine, 12(46), 160–164. https://doi.org/10.4103/0973-1296.177903