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    Article

    Antioxidant Activity of Water‑soluble Polysaccharides from Brasenia schreberi

    Huiwen Xiao1★, Xueru Cai1★, Yijun Fan1, Aoxue Luo1★★ Corresponding author

    1. 1Department of Landscape Plants, Sichuan Agriculture University, 611 830 Sichuan, China.

    CORRESPONDENCE

    Huiwen Xiao

    Department of Landscape Plants, Sichuan Agriculture University, 611 830 Sichuan, China.

    aoxueluo@sina.com

    Received: 30-05-2015; Revised: 02-09-2015.

    Volume 12, Issue 47 · pp. 193–197 · PUBLISHED 14 July 2016 · DOI: 10.4103/0973-1296.186343

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objective: In order to investigate the antioxidant activities of polysaccharides (BPL-1 and BPL-2), one of the most important functional constituents in Brasenia schreberi was isolated from the external mucilage of B. schreberi (BPL-1) and the plant in vivo (BPL-2). This paper examines the relationship between the content of sulfuric radicals and uronic acid in BPL and the antioxidant activity of BPL. Materials and Methods:The free radicals, 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) and 1,1-diphnyl-2-picrylhydrazyl (DPPH-), were used to determine the antioxidant activity of BPL. The Fourier-transform infrared spectroscopy of BPL-1 and BPL-2 revealed typical characteristics of polysaccharides. Results: The two sample types had different contents. This was proved by their different adsorption peak intensities. The IC50 values of BPL-1 (31.189 mg/ml) and BPL-2 (1.863 mg/ml) showed significant DPPH radical scavenging activity. Based on the quantification of ABTS radical scavenging, the IC50 value of BPL-1 (5.460 mg/ml) was higher than that of BPL-2 (0.239 mg/ml). Therefore, in terms of the reducing power, the IC50 value of BPL-1 was too high to determine, and the IC50 value of BPL-2 was found to be 50.557 mg/ml. Hence, the antioxidant activity and total reducing power were high, and they were greater in BPL-2 than in BPL-1. In addition, BPL-2 was found to have more sulfuric radicals and uronic acid than BPL-1. Conclusion: The contents of sulfuric radicals and uronic acid are significantly correlated to the antioxidant activity and reducing power of BPL; the more sulfuric radicals and uronic acid, the more antioxidant activity and reducing power BPL has.

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      Xiao, H., Cai, X., Fan, Y., & Luo, A. (2016). Antioxidant Activity of Water‑soluble Polysaccharides from Brasenia schreberi. Pharmacognosy Magazine, 12(47), 193–197. https://doi.org/10.4103/0973-1296.186343