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    Ursolic Acid, a Natural Pentacylcic Triterpene from Ochrosia elliptica and Its Role in The Management of Certain Neglected Tropical Diseases

    Rola M. Labib1,2★, Sherif S. Ebada1, Fadia S. Youssef1, Mohamed L. Ashour1, Samir A. Ross2,3★ Corresponding author

    1. 1Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.
    2. 2National Center for Natural Products Research, USA.
    3. 3Department of BioMolecular Sciences, School of Pharmacy, University of Mississippi, University, MS, USA.

    CORRESPONDENCE

    Rola M. Labib

    Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.

    rolamilad@pharma.asu.edu.eg

    Received: 02-03-2016; Revised: 22-02-2016; Accepted: 22-02-2016.

    Volume 12, Issue 48 · pp. 319–25 · PUBLISHED 13 October 2016 · DOI: 10.4103/0973-1296.192207

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Leishmaniasis and African trypanosomiasis are recognized as the leading causes of mortality and morbidity with the greatest prevalence in the developing countries. They affect more than one billion of the poorest people on the globe. Objective: To find a cheap, affordable, safe, and efficacious antileshmanial and antitrypanosomal natural drug and to elucidate its probable mode of action. Materials and Methods: Phytochemical investigation of the non-polar fraction of the methanol extract of leaves of Ochrosia elliptica Labill. (Apocyanaceae) resulted in the isolation of ursolic acid, which was unambiguously determined based on HR-ESI-FTMS, extensive 1D and 2D NMR spectroscopy. It was further tested for its cytotoxicity, antimicrobial, antimalarial, antileishmanial, and trypanocidal potency. in-silico molecular modeling studies were conducted on six vital parasitic enzymes including farnesyl diphosphate synthase, N-myristoyl transferase, pteridine reductase 1, trypanothione reductase, methionyl-tRNA synthetase, and inosine–adenosine–guanosine nucleoside hydrolase to discover its potential mode of action as antitrypanosomal and antileishmanial agent. Results: Ursolic acid displayed considerable antitrypanosomal and antileishmanial activities with IC50 values ranging between 1.53 and 8.79 μg/mL. It showed superior antitrypanosomal activity as compared to the standard drug difluoromethylornithine (DFMO), with higher binding affinities towards trypanothione reductase and pteridine reductase 1. It displayed free binding energy of -30.73 and -50.08 kcal/mole towards the previously mentioned enzymes, respectively. In addition, ursolic acid exhibited considerable affinities to farnesyl diphosphate synthase, N-myristoyl transferase and methionyl-tRNA synthetase with free binding energies ranging from -42.54 to -63.93 kcal/mole. Conclusion: Ursolic acid offers a safe, effective and cheap antitrypanosomal and antileishmanial candidate acting on several key parasitic enzymes.

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      Labib, R. M., Ebada, S. S., Youssef, F. S., Ashour, M. L., & Ross, S. A. (2016). Ursolic Acid, a Natural Pentacylcic Triterpene from Ochrosia elliptica and Its Role in The Management of Certain Neglected Tropical Diseases. Pharmacognosy Magazine, 12(48), 319–25. https://doi.org/10.4103/0973-1296.192207