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    Article

    Trichosanthes kirilowii Exerts Androgenic Activity via Regulation of PSA and KLK2 in 22Rv1 Prostate Cancer Cells

    Soo-Jin Jeong1,2, Ji-Yoon Choi3, Mi-Sook Dong3, Chang-Seob Seo4, Hyeun-Kyoo Shin4★★ Corresponding author

    1. 1KM Convergence Research Division, Korea Institute of Oriental Medicine, Deajeon, Republic of Korea.
    2. 2Korean Medicine Life Science, University of Science and Technology, Daejeon, Republic of Korea.
    3. 3School of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
    4. 4K-herb Research Center, Korea Institute of Oriental Medicine, Deajeon, Republic of Korea.

    CORRESPONDENCE

    Hyeun-Kyoo Shin

    K-herb Research Center, Korea Institute of Oriental Medicine, Deajeon, Republic of Korea.

    hkshin@kiom.re.kr

    Received: 19-11-2015; Revised: 09-02-2016; Accepted: 09-02-2016.

    Volume 13, Issue 49 · pp. 153–8 · PUBLISHED 6 January 2017 · DOI: 10.4103/0973-1296.197657

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: The androgen comprises a group of hormones that play roles in male reproductive activity as well as personal characteristics. Objective: We investigated the androgenic activity of various herbal medicines in human prostate cancer 22Rv1 cells. Materials and Methods: Herbal extracts of Trichosanthes kirilowii (TK), Asarum sieboldii (AS), Sanguisorba officinalis (SO), and Xanthium strumarium (XS) were selected to have androgenic effects based on a preliminary in vitro screening system. Results: TK, AS, SO, and XS enhanced the proliferation of 22Rv1 cells without having cytotoxic effects. All tested herbal extracts increased androgen receptor (AR)-induced transcriptional activity in the absence or presence of dihydrotestosterone (DHT). In an AR-binding assay, TK, but not AS, SO, or XS, produced a significant inhibition of AR binding activity, indicating it has androgenic activity. Additionally, TK treatment positively regulated mRNA expression of the AR-related molecular targets prostate-specific antigen (PSA) and kallikrein 2 (KLK2) compared with untreated control. Conclusion: Taken together, TK-enhanced AR-mediated transcriptional activity might be an attractive candidate drug for treating androgen-related diseases.

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      Jeong, S., Choi, J., Dong, M., Seo, C., & Shin, H. (2017). Trichosanthes kirilowii Exerts Androgenic Activity via Regulation of PSA and KLK2 in 22Rv1 Prostate Cancer Cells. Pharmacognosy Magazine, 13(49), 153–8. https://doi.org/10.4103/0973-1296.197657