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    Preparation of Ginseng Extract with Enhanced Levels of Ginsenosides Rg1 and Rb1 Using High Hydrostatic Pressure and Polysaccharide Hydrolases

    Sasikumar Arunachalam Palaniyandi1,2, Joo-Won Suh2,3★, Seung Hwan Yang4★★ Corresponding author

    1. 1Interdisciplinary Program of Biomodulation, Myongji University, Cheoin-gu, Yongin, Gyeonggi-Do, Korea.
    2. 2Center for Nutraceutical and Pharmaceutical Materials, Myongji University, Cheoin-gu, Yongin, Gyeonggi-Do, Korea.
    3. 3Division of Bioscience and Bioinformatics, College of Natural Science, Myongji University, Cheoin-gu, Yongin, Gyeonggi-Do, Korea.
    4. 4Department of Biotechnology, Chonnam National University, Daehak-ro, Yeosu-si, Jeollanam-do, Korea.

    CORRESPONDENCE

    Joo-Won Suh

    Center for Nutraceutical and Pharmaceutical Materials, Myongji University, Cheoin-gu, Yongin, Gyeonggi-Do, Korea.

    ymichigan@jnu.ac.kr

    Received: 22-09-2015; Revised: 12-11-2015; Accepted: 12-11-2015.

    Volume 13, Issue 49s · pp. 142–7 · PUBLISHED 7 April 2017 · DOI: 10.4103/0973-1296.203992

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Ginsenosides are the principal components responsible for the pharmacological activities of ginseng. Ginsenosides Rg1 and Rb1 are the major compounds recognized as marker substances for quality control of ginseng-based products. These major compounds can be transformed to several pharmacologically active minor ginsenosides by chemical, microbial, and enzymatic means. Materials and Methods: In the present study, a combination of polysaccharide hydrolases and high hydrostatic pressure (HHP) were used to extract ginseng saponins enriched with ginsenosides Rg1 and Rb1. Temperature, pH, time, ginseng-to-water ratio, and pressure were optimized to obtain the maximum amount of Rg1 and Rb1 in the resulting extract using commercial polysaccharide hydrolases. Results: This study showed that treatment with a combination of cellulase, amylase, and pectinase at 100 MPa pressure, pH 4.8, and 45°C for 12 h resulted in higher Rg1 and Rb1 levels in the extract. Conclusion: This study describes a cheap and ecofriendly method for preparing ginseng extract enriched with Rg1 and Rb1.

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      Palaniyandi, S. A., Suh, J., & Yang, S. H. (2017). Preparation of Ginseng Extract with Enhanced Levels of Ginsenosides Rg1 and Rb1 Using High Hydrostatic Pressure and Polysaccharide Hydrolases. Pharmacognosy Magazine, 13(49s), 142–7. https://doi.org/10.4103/0973-1296.203992