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    Article

    Cytotoxic Metabolites from Callyspongia siphonella Display Antiproliferative Activity by Inducing Apoptosis in HCT-116 Cells

    Tariq R.A. Sobahi1, Seif-Eldin N. Ayyad1,2, Ahmed Abdel-Lateff3,4★, Mardi M. Algandaby5, Hajer S. Alorfi1, Ashraf B. Abdel-Naim6★ Corresponding author

    1. 1Department of Chemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
    2. 2Department of Chemistry, Faculty of Science, Damietta University, Damietta, Egypt.
    3. 3Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia.
    4. 4Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia, Egypt.
    5. 5Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
    6. 6Department of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia.

    CORRESPONDENCE

    Ahmed Abdel-Lateff

    Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia.

    aabdellateff@kau.edu.sa

    Received: 02-08-2016; Revised: 08-09-2016; Accepted: 08-09-2016.

    Volume 13, Issue 49s · pp. 37–40 · PUBLISHED 7 April 2017 · DOI: 10.4103/0973-1296.203970

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objectives: To evaluate the antiproliferative effect of the isolated metabolites from Callyspongia siphonella. Methods: Different chromatographic methods have been done on the organic extract of the marine sponge aiming at isolating the bioactive metabolites. The cytotoxicity of the isolated compounds has been evaluated against the human colorectal cancer cell line; HCT-116, employing SRB assay. The flow cytometry assay was applied to measure the cell cycle analysis. Results: Six metabolites (1–6) were obtained. The compounds 4–6 exhibited IC50 values (µM ± SD) of 95.80± 1.34, 14.8 ± 2.33, and 19.8 ± 3.78, respectively. Cell cycle distribution analysis revealed that sipholenol A (5) and sipholenol L (6) induced G2/M and S phase arrest with concomitant increase in the pre-G cell population. Furthermore, 5 and 6 increased the nuclear expression of the pro-apoptotic protein-cleaved caspase-3 that effectively drives cellular apoptosis via caspase-3-dependent pathway. Conclusions: The antiproliferative activity of 5 and 6 can be recognized, at least partly, due to their ability to induce cellular apoptosis.

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      Sobahi, T. R., Ayyad, S. N., Abdel-Lateff, A., Algandaby, M. M., Alorfi, H. S., & Abdel-Naim, A. B. (2017). Cytotoxic Metabolites from Callyspongia siphonella Display Antiproliferative Activity by Inducing Apoptosis in HCT-116 Cells. Pharmacognosy Magazine, 13(49s), 37–40. https://doi.org/10.4103/0973-1296.203970