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    Article

    Hepatoprotective Flavonoids in Opuntia ficus‑indica Fruits by Reducing Oxidative Stress in Primary Rat Hepatocytes

    Jung Wha Kim1★, Tae Bum Kim1★, Hyun Woo Kim1, Sang Wook Park2, Hong Pyo Kim3, Sang Hyun Sung1★★ Corresponding author

    1. 1Department of Pharmacy, College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University.
    2. 2Department of Agriculture, College of Agriculture and Life Sciences, Seoul National University, Seoul.
    3. 3Department of Pharmacy, College of Pharmacy, Ajou University, Suwon, Republic of Korea Submitted: 09-05-2016 Revised: 11-07-2016 Published: 19-07-2017.

    CORRESPONDENCE

    Jung Wha Kim

    Department of Pharmacy, College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University.

    shsung@snu.ac.kr

    Received: 09-05-2016; Revised: 11-07-2016.

    Volume 13, Issue 51 · pp. 472–476 · PUBLISHED 19 July 2017 · DOI: 10.4103/pm.pm_232_16

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Liver disorder was associated with alcohol consumption caused by hepatic cellular damages. Opuntia ficus‑indica fruit extracts (OFIEs), which contain betalain pigments and polyphenols including flavonoids, have been introduced as reducing hangover symptoms and liver protective activity. Objective: To evaluate hepatoprotective activity of OFIEs and isolated compounds by high‑speed countercurrent chromatography (HSCCC). Materials and Methods: The extract of O. ficus‑indica fruits was fractionated into methylene chloride and n‑butanol. The n‑butanol fraction was isolated by HSCCC separation (methylene chloride‑methanol‑n-butanol‑water, 5:4:3:5, v/v/v/v). The hepatoprotective activity of OFIEs and isolated compounds was evaluated on rat primary hepatocytes against ethanol‑induced toxicity. Antioxidative parameters such as glutathione reductase and glutathione peroxidase (GSH‑Px) enzymes and the GSH content were measured. Results: Two flavonoids, quercetin 3‑O‑methyl ester (1) and (+)‑taxifolin, and two flavonoid glycosides, isorhamnetin 3‑O‑β‑d‑glucoside (3) and narcissin (4), were isolated from the n‑butanol fraction by HSCCC separation. Among them, compound 2 significantly protected rat primary hepatocytes against ethanol exposure by preserving antioxidative properties of GR and GSH‑Px. Conclusions: OFIEs and (+)‑taxifolin were suggested to reduce hepatic damage by alcoholic oxidative stress.

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      Kim, J. W., Kim, T. B., Kim, H. W., Park, S. W., Kim, H. P., & Sung, S. H. (2017). Hepatoprotective Flavonoids in Opuntia ficus‑indica Fruits by Reducing Oxidative Stress in Primary Rat Hepatocytes. Pharmacognosy Magazine, 13(51), 472–476. https://doi.org/10.4103/pm.pm_232_16