Anticancer Potential of Steviol in MCF-7 Human Breast Cancer Cells
Ena Gupta1★, Shweta Kaushik2, Shalini Purwar1, Ramesh Sharma2, Anil K. Balapure2, Shanthy Sundaram1★ Corresponding author
- 1Centre of Biotechnology, University of Allahabad, Allahabad, India.
- 2Division of Biochemistry, Central Drug Research Institute, Lucknow, Uttar Pradesh, India.
CORRESPONDENCE
Ena Gupta
Centre of Biotechnology, University of Allahabad, Allahabad, India.
Received: 26-01-2017; Revised: 21-02-2017.
Volume 13, Issue 51 · pp. 345–350 · PUBLISHED 19 July 2017 · DOI: 10.4103/pm.pm_29_17
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ABSTRACT
Objective: This study aimed to investigate the cytotoxicity, apoptosis induction, and mechanism of action of steviol on human breast cancer cells (Michigan Cancer Foundation‑7 [MCF‑7]). Materials and Methods: Sulforhodamine‑B assay was performed to analyze cytotoxic potential of Steviol whereas flow cytometer was used to analyze cell cycle, apoptosis, and reactive oxygen species generation. Results: Studying the viability of cells confirms the IC50 of Steviol in MCF‑7 cells which was 185 µM. The data obtained from fluorescence‑activated cell sorter analysis reveal Steviol‑mediated G2/M‑phase arrest (P < 0.05) in addition to the presence of evident sub‑G0/G1 peak (P < 0.05) in the MCF‑7 cells, signifying the ongoing apoptosis. Conclusion: Thus, results suggest that induction of apoptosis in MCF‑7 cells was due to dose‑dependent effect of Steviol. Our first in vitro findings indicate Steviol as a promising candidate for the treatment of breast cancer.
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Gupta, E., Kaushik, S., Purwar, S., Sharma, R., Balapure, A. K., & Sundaram, S. (2017). Anticancer Potential of Steviol in MCF-7 Human Breast Cancer Cells. Pharmacognosy Magazine, 13(51), 345–350. https://doi.org/10.4103/pm.pm_29_17