The Effect of Thymoquinone on Nuclear Factor Kappa B Levels and Oxidative DNA Damage on Experimental Diabetic Rats
Ayşe Usta1, Semiha Dede2★★ Corresponding author
- 1Department of Chemistry, Faculty of Science, Yuzuncu Yil University, Turkey.
- 2Department of Biochemistry, Yuzuncu Yil University, Faculty of Veterinary Medicine, Van, Turkey.
CORRESPONDENCE
Semiha Dede
Department of Biochemistry, Yuzuncu Yil University, Faculty of Veterinary Medicine, Van, Turkey.
Received: 10-04-2017; Revised: 17-05-2017.
Volume 13, Issue 51s · pp. S458–S461 · PUBLISHED 11 October 2017 · DOI: 10.4103/pm.pm_134_17
View on Pharmacogn. Mag. original site ↗
ABSTRACT
Background: Thymoquinone (TQ), the basic bioactive phytochemical constituent of seed oil of Nigella sativa, is one of these herbal drugs known for antidiabetic effects. This study was carried out to assess the effects of the possible role of TQ on nuclear factor kappa B (NF‑κB) and oxidative DNA damage levels in experimental diabetic rats. Materials and Methods: Twenty‑eight male Wistar Albino rats (200–250 g) were used as experimental subjects. The rats were divided into four groups, including the control, control supplemented with TQ (CT), diabetic (D), and diabetic supplemented with TQ (DT), each containing seven rats. The D and the DT groups were treated with 45 mg/kg streptozotocin (STZ) (intraperitoneal). TQ was administered 30 mg/kg/day for 21 days by oral gavage in the DT and the T groups. Results: It was determined that glucose, glycosylated hemoglobin (HbA1c) levels and alanine aminotransferase, aspartate aminotransferase, and gamma‑glutamyl transpeptidase activities were decreased significantly and approached the control group in the DT group after TQ supplement (P < 0.05). Urea levels were the lowest in CT (P < 0.05). Oxidative DNA damage (8 hydroxy‑2‑deoxyguanosine) was increased in both of the diabetic groups (D and DT). The NF‑κB levels were the highest in Group D (P < 0.05). Conclusion: It was observed that increased glucose and HbA1c levels and the indicators of liver and kidney damages were decreased significantly after TQ supplementation. Oxidative DNA damage and NF‑κB levels were increased in the diabetic group, and TQ administration caused a statistically insignificant reduction.
KEYWORDS
REFERENCES
As publishedShowing references and in-text citations exactly as published.
Cite this article
SELECT FORMAT
Usta, A., & Dede, S. (2017). The Effect of Thymoquinone on Nuclear Factor Kappa B Levels and Oxidative DNA Damage on Experimental Diabetic Rats. Pharmacognosy Magazine, 13(51s), S458–S461. https://doi.org/10.4103/pm.pm_134_17