Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy MagazineNOW IN
    Article

    Simultaneous Determination of Ten Constituents in Chaiqin Qingning Capsule by High‑performance Liquid Chromatography Coupled with Triple‑quadrupole Mass Spectrometry

    Ting Yu Li2,3, Xiao Kui Huo3, Lu Zheng4, Chao Wang3, Hai Jian Cong4, Ting Xiang5, Lin Zhang3★, Bao Jing Zhang3, Shan Shan Huang3, Bin Wu6★, Xin Yu Li7★★ Corresponding author

    1. 1Department of Pharmacy, Dalian Children’s Hospital, China.
    2. 2Academy of Integrative Medicine, College of Pharmacy, Dalian Medical University, China.
    3. 3The Department of New Drug, Shanghai Haini Pharmaceutical Co. Ltd., Shanghai 310000, China.
    4. 4The Department of New Drug, Yangtze River Pharmaceutical Group, Taizhou 225321, China.
    5. 5Shanghai Center for Drug Evaluation and Inspection, SHFDA, Shanghai 201203, China.
    6. 6Department of Endocrinology, Dalian Municipal Central Hospital, Affiliated of Dalian Medical University, Dalian, China.

    CORRESPONDENCE

    Lin Zhang

    The Department of New Drug, Shanghai Haini Pharmaceutical Co. Ltd., Shanghai 310000, China.

    zhl8247@163.com

    Received: 08-03-2017; Revised: 21-03-2017.

    Volume 13, Issue 52 · pp. 566–570 · PUBLISHED 13 November 2017 · DOI: 10.4103/pm.pm_81_17

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Chaiqin Qingning Capsule (CQQNC) was a prescription of Traditional Chinese Medicine with the effects of clearing away heat and removing toxin, harmonizing the exterior and interior, it was widely used in Asian, for example, China and Japan, different batches of the raws materials and different processing time may be the vital factor which raised a challenge to control the quality of the CQQNC. Experimental Methods: In this experiment, a high‑performance liquid chromatography‑mass spectrometry/MS (HPLC‑MS/MS) method was developed to simultaneously determine ten bioactive components for the quality control of CQQNC. Chromatographic separation was achieved using an XBridge BEH C18 column (150 mm × 4.6 mm, 2.5 µm) with a mobile phase composed of 10 mm aqueous ammonium acetate and acetonitrile using a gradient elution in 20 min. This study was conducted by multiple reaction monitoring mode through electrospray ionization resource with a negative ionization mode. Results: The established method was validated with good performance of precision, accuracy, stability, and reproducibility and was utilized to simultaneously quantify ten constituents of CQQNC obtained from seven different batches. Conclusion: It is the first time to report the rapid and simultaneous analysis of the ten compounds in CQQNC by HPLC‑MS/MS and apply to determine 10 constituents in 7 batches of CQQNC bought from drug store in china. This method could be considered as good quality criteria to control the quality of CQQNC.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Li, T. Y., Huo, X. K., Zheng, L., Wang, C., Cong, H. J., Xiang, T., Zhang, L., Zhang, B. J., Huang, S. S., Wu, B., & Li, X. Y. (2017). Simultaneous Determination of Ten Constituents in Chaiqin Qingning Capsule by High‑performance Liquid Chromatography Coupled with Triple‑quadrupole Mass Spectrometry. Pharmacognosy Magazine, 13(52), 566–570. https://doi.org/10.4103/pm.pm_81_17