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    Identification of Hepatoprotective Constituents in Limonium tetragonum and Development of Simultaneous Analysis Method using High‑performance Liquid Chromatography

    Jae Sun Lee1, Yun Na Kim1, Na‑Hyun Kim2, Jeong‑Doo Heo2, Min Hye Yang3, Jung‑Rae Rho4★, Eun Ju Jeong1★★ Corresponding author

    1. 1Department of Agronomy and Medicinal Plant Resources, Gyeongnam National University of Science and Technology, Jinju 52725.
    2. 2Gyeongnam Department of Environment and Toxicology, Korea Institute of Toxicology, Gyeongnam 52834.
    3. 3College of Pharmacy, Pusan National University, Busan 46241.
    4. 4Department of Oceanography, Kunsan National University, Jeonbuk 54150, Republic of Korea.

    CORRESPONDENCE

    Jung‑Rae Rho

    Department of Oceanography, Kunsan National University, Jeonbuk 54150, Republic of Korea.

    jrrho@kunsan.ac.kr

    Received: 24-10-2016; Revised: 07-12-2016.

    Volume 13, Issue 52 · pp. 535–541 · PUBLISHED 13 November 2017 · DOI: 10.4103/pm.pm_477_16

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Limonium tetragonum, a naturally salt‑tolerant halophyte, has been studied recently and is of much interest to researchers due to its potent antioxidant and hepatoprotective activities. Objective: In the present study, we attempted to elucidate bioactive compounds from ethyl acetate (EtOAc) soluble fraction of L. tetragonum extract. Furthermore, the simultaneous analysis method of bioactive EtOAc fraction of L. tetragonum has been developed using high‑performance liquid chromatography (HPLC). Materials and Methods: Thirteen compounds have been successfully isolated from EtOAc fraction of L. tetragonum, and the structures of 1–13 were elucidated by extensive one‑dimensional and two‑dimensional spectroscopic methods including 1H‑NMR, 13C‑NMR, 1H‑1H COSY, heteronuclear single quantum coherence, heteronuclear multiple bond correlation, and nuclear Overhauser effect spectroscopy. Hepatoprotection of the isolated compounds against liver fibrosis was evaluated by measuring inhibition on hepatic stellate cells (HSCs) undergoing proliferation. Results: Compounds 1–13 were identified as gallincin (1), apigenin‑3‑O‑β‑D‑galactopyranoside (2), quercetin (3), quercetin‑3‑O‑β‑D‑galactopyranoside (4), (−)‑epigallocatechin (5), (−)‑epigallocatechin‑3‑gallate (6), (−)‑epigallocatechin‑3‑(3’’‑O‑methyl) gallate (7), myricetin‑3‑O‑β‑D‑galactopyranoside (8), myricetin‑3‑O‑(6’’‑O‑g alloyl)‑β‑D‑galactopyranoside (9), myricetin‑3‑O‑α‑L‑rhamnopyranoside (10), myricetin‑3‑O‑(2’’‑O‑galloyl)‑α‑L‑rhamnopyranoside (11), myricetin‑3‑O‑(3’’‑O ‑galloyl)‑α‑L‑rhamnopyranoside (12), and myricetin‑3‑O‑α‑L‑arabinopyranos ide (13), respectively. All compounds except for 4, 8, and 10 are reported for the first time from this plant. Conclusion: Myricetin glycosides which possess galloyl substituent (9, 11, and 12) showed most potent inhibitory effects on the proliferation of HSCs.

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      Lee, J. S., Kim, Y. N., Kim, N., Heo, J., Yang, M. H., Rho, J., & Jeong, E. J. (2017). Identification of Hepatoprotective Constituents in Limonium tetragonum and Development of Simultaneous Analysis Method using High‑performance Liquid Chromatography. Pharmacognosy Magazine, 13(52), 535–541. https://doi.org/10.4103/pm.pm_477_16