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    Hispidulin-7-O-Neohesperidoside from Cirsium japonicum var. ussuriense Attenuates the Production of Inflammatory Mediators in LPS-Induced Raw 264.7 Cells and HT-29 Cells

    Jong Cheol Park1, Hyunji Yoo2, Cho Een Kim2, Sun-Yup Shim2,3, Mina Lee2,4★★ Corresponding author

    1. 1Department of Oriental Medicine Resources, College of Pharmacy, Sunchon National University, Jungangno, Suncheon-si, Jeonnam.
    2. 2College of Pharmacy, Sunchon National University, Jungangno, Suncheon-si, Jeonnam.
    3. 3Research Institute of Life and Pharmaceutical Sciences, Jungangno, Suncheon-si, Jeonnam, Korea.
    4. 4Suncheon Research Center for Natural Medicines, 255 Jungangno, Suncheon-si 57922, Jeonnam, Republic of Korea.

    CORRESPONDENCE

    Mina Lee

    College of Pharmacy, Sunchon National University, Jungangno, Suncheon-si, Jeonnam.

    minalee@sunchon.ac.kr

    Received: 26-06-2016; Revised: 18-07-2016.

    Volume 13, Issue 52 · pp. 707–711 · PUBLISHED 13 November 2017 · DOI: 10.4103/0973-1296.218116

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Inflammatory bowel disease (IBD) is characterized by chronic inflammation of the gastrointestinal tract and involves secretion of inflammatory mediators. The flavone diglycoside hispidulin-7-O- neohesperidoside (HN) isolated from the methanolic extract of aerial parts of Cirsium japonicum var. ussuriense, but its pharmacologic activities, with the exception of alleviation of alcohol toxicity, have not been investigated to date. Objective: The aim of the present study was to investigate the anti-inflammatory activities of HN for the treatment of chronic inflammatory illnesses, including IBD. Materials and Methods: In lipopolysaccharide (LPS)-induced RAW264.7 cells and HT-29 cells, the effects of HN on cell viability and nitric oxide (NO) production were examined via MTT assay and the Griess reaction, respectively. The expression levels of interleukin (IL)-1α, IL-8, and tumor necrosis factor (TNF)-α and inducible nitric oxide synthase (iNOS) protein levels were measured by enzyme-linked immunosorbent assay and Western blotting, respectively. Results: HN concentration- dependently inhibited NO production in LPS-induced RAW 264.7 cells. Treatment with HN considerably downregulated the levels of the pro- inflammatory cytokines, IL-1 and TNF-α and the iNOS protein level in LPS- induced RAW 264.7 cells. Furthermore, HN inhibited the production of the chemotactic cytokine, IL-8, in LPS-induced HT-29 cells. Conclusion: HN has potential as an anti-inflammatory agent to prevent and/or treat IBD. SUMMARY • Hispidulin-7-O-neohesperidoside (HN) is flavone diglycoside isolated from the methanolic extract of aerial parts of Cirsium japonicum var. ussuriense. • HN concentration-dependently inhibited NO production and considerably downregulated the levels of the proinflammatory cytokines, IL-1β and TNF-α, and the iNOS protein level in LPS-induced RAW 264.7 cells. • HN inhibited the production of the chemotactic cytokine, IL-8, in LPS-induced HT-29 cells. • HN has potential as an anti-inflammatory agent to prevent and/or treat IBD.

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      Park, J. C., Yoo, H., Kim, C. E., Shim, S., & Lee, M. (2017). Hispidulin-7-O-Neohesperidoside from Cirsium japonicum var. ussuriense Attenuates the Production of Inflammatory Mediators in LPS-Induced Raw 264.7 Cells and HT-29 Cells. Pharmacognosy Magazine, 13(52), 707–711. https://doi.org/10.4103/0973-1296.218116