Capsaicin Reverses the Inhibitory Effect of Licochalcone A/β-Arbutin on Tyrosinase Expression in B16 Mouse Melanoma Cells
Jun-Hui Hong1, Huo-Ji Chen2, Shi-Jian Xiang3, Si-Wei Cao3, Bai-Chao An3, Shi-Fa Ruan3, Bin Zhang3, Li-Dong Weng3, Hong-Xia Zhu3, Qiang Liu3★★ Corresponding author
- 1Department of Chinese medicine preparation, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province 510515, P R China.
- 2Department of Pharmacy, The affiliated hospital of Qingdao University, Qingdao, P R China.
- 3Department of Pediatrics, Hospital of Integrated Chinese and Western Medcine, Southern Medical University, Guangzhou, Guangdong Province 510515, P R China.
CORRESPONDENCE
Qiang Liu
Department of Pediatrics, Hospital of Integrated Chinese and Western Medcine, Southern Medical University, Guangzhou, Guangdong Province 510515, P R China.
Received: 10-03-2017; Revised: 25-04-2017; Accepted: 20-02-2018.
Volume 14, Issue 53 · pp. 110–5 · PUBLISHED 20 February 2018 · DOI: 10.4103/pm.pm_103_17
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ABSTRACT
Introduction: Melanin is synthesized by melanocytes, which are located in the basal layer of the skin. After synthesis, melanin is further deposited on the surface of the skin to form black spots or chloasma. Tyrosinase is a rate‑limiting enzyme that plays an important role in melanogenesis. Currently, there are many drugs that inhibit tyrosinase expression to further reduce melanogenesis. Nevertheless, some of these could reverse the pharmacological effect of other drugs, when used simultaneously. Materials and Methods: B16 mouse melanoma cells were treated with the tyrosinase inhibitors licochalcone A and β‑arbutin, alone or in combination with capsaicin, an alkaloid found in peppers. Cytotoxicity, melanin content, and tyrosinase activity and expression were determined. Results: Licochalcone A/β‑arbutin inhibited tyrosinase expression and further hindered melanin synthesis when applied individually to B16 mouse melanoma cells. However, licochalcone A/β‑arbutin combined with 50 μmol/L capsaicin enhanced the expression of tyrosinase in these cells and further increased melanin content. Conclusion: Our data implied that capsaicin could reverse the inhibitory effect of licochalcone A/β‑arbutin on tyrosinase expression in B16 mouse melanoma cells.
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Hong, J., Chen, H., Xiang, S., Cao, S., An, B., Ruan, S., Zhang, B., Weng, L., Zhu, H., & Liu, Q. (2018). Capsaicin Reverses the Inhibitory Effect of Licochalcone A/β-Arbutin on Tyrosinase Expression in B16 Mouse Melanoma Cells. Pharmacognosy Magazine, 14(53), 110–5. https://doi.org/10.4103/pm.pm_103_17