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    Article

    Antioxidant and Hemolysis Protective Effects of Polyphenol-Rich Extract from Mulberry Fruits

    Palanigounder Ganeshan Ajay Krishna1, Thasma Raman Sivakumar1, Chao Jin1, Shao-Hui Li1, Yu-Jie Weng1, Juan Yin1, Jun-Qiang Jia1,2, Chu-Yan Wang3, Zhong-Zheng Gui1,2★★ Corresponding author

    1. 1Lab of Hi-Tech Processing for Sericultural Resources, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, China.
    2. 2Lab of Hi-Tech Processing for Sericultural Resources, Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China.
    3. 3Department of Biological and Environmental Engineering, Hefei University, Hefei, Anhui 230601, China.

    CORRESPONDENCE

    Zhong-Zheng Gui

    Lab of Hi-Tech Processing for Sericultural Resources, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, China.

    srizzgui@hotmail.com

    Received: 04-11-2016; Revised: 23-11-2016; Accepted: 23-11-2016.

    Volume 14, Issue 53 · pp. 103–9 · PUBLISHED 20 February 2018 · DOI: 10.4103/pm.pm_491_16

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Mulberry fruits are a superior source of polyphenol, especially anthocyanins that contribute potentially to the beneficial effects which include reducing the risk of cardiovascular diseases and cancers with antioxidant, anti‑inflammatory, and chemoprotective properties. Objectives: In this study, purification of the polyphenol‑rich extract from mulberry fruit (MPE) was purified and assessed the activities of antioxidant and hemolysis protective in vivo and in vitro. Materials and Methods: Antioxidant activities in vitro was measured by quantifying its 2,2‑diphenyl‑1‑picrylhydrazyl (DPPH) scavenging activity, reducing power and Fe2+‑chelating ability. MPE was purified by high‑pressure liquid chromatography (HPLC) and analyzed individual polyphenols using liquid chromatography–mass spectrometry (LC‑MS)/MS. Results: The total polyphenol content was 147.69 ± 0.02 mg gallic acid equivalents (GAE)/g dried weight (DW) in the extract and 403.55 ± 0.02 mg GAE/g DW in the purified extract. Further identification by HPLC‑ultraviolet‑visible and LC‑MS/MS analysis indicated in MPE, an anthocyanin compound, cyanidin‑3‑O‑glucoside. With regard to in vitro assays, MPE possessed antioxidant effect, especially in Fe2+ chelating ability with an IC50 value of 1.016 mg/mL. The protective effects on mouse red blood cell hemolysis and lipid peroxidation ex vivo were dose and time dependent. Conclusion: It indicates that MPE could be a good candidate for future biomedical applications to promote human health with limited side effects.

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      Krishna, P. G. A., Sivakumar, T. R., Jin, C., Li, S., Weng, Y., Yin, J., Jia, J., Wang, C., & Gui, Z. (2018). Antioxidant and Hemolysis Protective Effects of Polyphenol-Rich Extract from Mulberry Fruits. Pharmacognosy Magazine, 14(53), 103–9. https://doi.org/10.4103/pm.pm_491_16