Novel Method of Preparation and Activity Research on Arctigenin from Fructus Arctii
Enbo Cai1, Jiahong Han1, Limin Yang1★, Weiyuan Zhang1, Yan Zhao1, Qiulian Chen1, Meng Guo1, Xinhong He1★ Corresponding author
- 1Department of Traditional Chinese Medicine, College of Chinese Medicinal Material, Jilin Agricultural University, Changchun, Jilin Province 130118, China.
CORRESPONDENCE
Limin Yang
Department of Traditional Chinese Medicine, College of Chinese Medicinal Material, Jilin Agricultural University, Changchun, Jilin Province 130118, China.
Received: 20-11-2016; Revised: 04-01-2017.
Volume 14, Issue 53 · pp. 87–94 · PUBLISHED 20 February 2018 · DOI: 10.4103/pm.pm_514_16
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ABSTRACT
Background: Arctigenin has many pharmacological activities with clinical significance and is derived from Arctium lappa L. However, the present extraction method is inefficient and does not have meaningful industrial production. Objective: A new method to directly prepare arctigenin was established by combining enzyme‑assisted extraction and central composite design. Arctigenin’s further pharmacological activity was also surveyed in vitro. Materials and Methods: β‑D‑Glucosidase, a food‑grade enzyme, was added directly to the fruits of A. lappa L. to hydrolyze the arctiin to arctigenin, and the obtained samples were subsequently subjected to ethanol (30%, v/v) extraction. The pharmacological activity of the extraction and arctigenin was determined by inhibiting acetylcholinesterase (AChE) and scavenging nitrite. Results: The factors investigated include the enzyme concentration (0.5%–2.5%), ultrasound time (10 min−3 0 min), and extraction temperature (30°C–50°C). From the analysis of the results by Design‑Expert (V8.0.6), the optimal extraction conditions were obtained: enzyme concentration (1.4%), ultrasound time (25 min), and extraction temperature (45°C). The highest yield of arctigenin, obtained under the optimal conditions was 6.39%, representing an increase of 28.15% compared to the reference extraction without enzyme processing. The IC50 values of the extraction and arctigenin, respectively, for inhibiting AChE were 0.572 mg/ml and 0.462 mg/ml, and those for nitrite‑scavenging were 34.571 mg/ml and 17.49 mg/ml. Conclusions: The results demonstrate that using an enzyme directly in the production is an effective means for extracting arctigenin from Fructus arctii. The extraction has the activities of inhibiting AChE and scavenging nitrite, probably because there has arctigenin in it. It is implied that the extraction and arctigenin could contribute to human health in clinical applications.
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Cai, E., Han, J., Yang, L., Zhang, W., Zhao, Y., Chen, Q., Guo, M., & He, X. (2018). Novel Method of Preparation and Activity Research on Arctigenin from Fructus Arctii. Pharmacognosy Magazine, 14(53), 87–94. https://doi.org/10.4103/pm.pm_514_16