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    Article

    In vitro Induction and Generation of Tetraploid Plants of Sophora tonkinensis Gapnep

    Kun Hua Wei2★, Jian Ping Xu3, Lin Xuan Li2, Jin Yuan Cai4, Jian Hua Miao2, Min Hui Li2,3★ Corresponding author

    1. 1Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, Guangxi, China.
    2. 2Inner Mongolia Research Center of Characteristic Medicinal Plants Cultivation and Protection Engineering Technology , Baotou Medical College, Baotou 014060, Inner Mongolia, China.
    3. 3Department of Food and Chemical Engineering, Lushan College of Guangxi University of Science and Technology, Liuzhou 545616, Guangxi, People’s Republic of, China.

    CORRESPONDENCE

    Kun Hua Wei

    Inner Mongolia Research Center of Characteristic Medicinal Plants Cultivation and Protection Engineering Technology , Baotou Medical College, Baotou 014060, Inner Mongolia, China.

    divinekh@163.com

    Received: 09-05-2017; Revised: 21-06-2017.

    Volume 14, Issue 54 · pp. 149–154 · PUBLISHED 10 April 2018 · DOI: 10.4103/pm.pm_170_17

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Sophora tonkinensis Gapnep. is an important medical plant in China. Early researches of S. tonkinensis were focused on rapid propagation and quality analysis of in vitro tissue culture plantlet, and still no research focuses on the plant breeding of and there were no excellent varieties for artificial cultivation of S. tonkinensis. Objective: To set up a method to generate and select the best varieties of S. tonkinensis by polyploid breeding after induction by colchicine treatment. Materials and Methods: The adventitious buds were submerged in different concentrations of aqueous colchicine solution for different lengths of time to induce polyploidy in the plants, and the induced buds were identified by root‑tip chromosome determination and leaf characteristics comparison. The contents of matrine and oxymatrine of radix ex rhizoma in 13 selected tetraploid lines were collected after 90 days in vitro rooting culture and were evaluated to provide evidence of good qualities of tetraploid S. tonkinensis. Results: The results showed that the highest percentage of tetraploid induction was 23.33% and occurred in the 0.2% (w/v) colchicine treatment for 30 h. Fifty lines of tetraploid plants were obtained and 12 of the 13 selected tetraploid lines exhibited higher productivity of total contents of matrine and oxymatrine when compared to controls. Conclusion: The data demonstrate that polyploidy induction can be beneficial for improving the medicinal value of S. tonkinensis.

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      Wei, K. H., Xu, J. P., Li, L. X., Cai, J. Y., Miao, J. H., & Li, M. H. (2018). In vitro Induction and Generation of Tetraploid Plants of Sophora tonkinensis Gapnep. Pharmacognosy Magazine, 14(54), 149–154. https://doi.org/10.4103/pm.pm_170_17