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    Insulinotropic and Cytoprotective Effect of L-theanine: An In vitro Dose Dependent Study

    Arpita Saha1★, Sirshendu Chatterjee2, Ananya Chatterjee1, Surmi Roy1, Nirmalya Roy3, Satinath Mukhopadhyay1★ Corresponding author

    1. 1Sandip K. Bandyopadhyay Departments of Biochemistry.
    2. 2Department of Biotechnology, Techno India University, Kolkata.
    3. 3Medicine, KPC Medical College, Kolkata.
    4. 4Department of Endocrinology, IPGME and R, Kolkata, West Bengal, College, Kolkata, India.

    CORRESPONDENCE

    Arpita Saha

    Sandip K. Bandyopadhyay Departments of Biochemistry.

    skb.teaboard@gmail.com

    Received: 15-12-2017; Revised: 25-01-2018.

    Volume 14, Issue 55s · pp. S36–S39 · PUBLISHED 28 June 2018 · DOI: 10.4103/pm.pm_595_17

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: L‑theanine is a natural bioactive amino acid present in tea (Camellia sinensis [L.] O. Kuntze) and consumed worldwide through tea decoction. Objective: The present work aims at evaluation of the insulin secretion and cytoprotective efficacy of L‑theanine, a bioactive amino acid present in tea, on pancreatic β‑cell line, RIN‑m5F. Methods: RIN m5F cell were treated with L-theanine alone or prior to hydrogen peroxide (200 um) treatment and subsequently cell viability, cellular morphology, insulin secretion and insulin gene expression were analyzed. Results: Studies have shown that L‑theanine dose dependently (0–300 µm) increases the β‑cell mass as well as increases insulin production by RIN‑m5F cell. It was also observed that pretreatment of the cell with L‑theanine partially protected the oxidative stress of β‑cells that were evident from cell viability, cellular morphology, and restoration of insulin‑secreting ability. Conclusion: Results suggest that L theanine is an insulinotropic agent as well as effective in giving partial protection to pancreatic β-cells in oxidatively stressed condition. L-theanine can be used in the prevention or treatment of diabetes.

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      Saha, A., Chatterjee, S., Chatterjee, A., Roy, S., Roy, N., & Mukhopadhyay, S. (2018). Insulinotropic and Cytoprotective Effect of L-theanine: An In vitro Dose Dependent Study. Pharmacognosy Magazine, 14(55s), S36–S39. https://doi.org/10.4103/pm.pm_595_17