T47D Cell‑inhibiting Indonesian Medicinal Plants and Active Constituents of Alpinia galanga Rhizome
Muhammad Da’i1★, Arini Fadhilah1, Juwita Rahmawati1, Alfian Mahardika Forentin1, Tepy Usia2, Maryati Maryati1★ Corresponding author
- 1Azis Saifudin Department of Pharmaceutical Chemistry, Universitas Muhammadiyah Surakarta, Jawa Tengah 57102, Indonesia.
- 2Research Center of National Drug and Food Control, Jakarta 10560, Indonesia.
CORRESPONDENCE
Muhammad Da’i
Azis Saifudin Department of Pharmaceutical Chemistry, Universitas Muhammadiyah Surakarta, Jawa Tengah 57102, Indonesia.
Received: 22-06-2017; Revised: 16-08-2017.
Volume 14, Issue 56 · pp. 359–363 · PUBLISHED 14 August 2018 · DOI: 10.4103/pm.pm_259_17
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ABSTRACT
Background: The screening of Indonesian medicinal plant extracts against T47D cell line and identifying active chemical constituents of Alpinia galanga rhizome were conducted. Materials and Methods: Thirty methanol extracts of Indonesian medicinal plants were screened for possible anti‑breast cancer. The T47D cell line was used as a target model. Chromatography techniques were used to purify the chemical constituents of A. galanga rhizome. Structural elucidation of isolated compounds was conducted by means of 1D and 2 D NMR (nuclear magnetic resonance) spectrometry. Their activities were also examined on MCF7, WiDr, and HeLa cell lines. Results: Five samples (A. galanga, Clonorchis sinensis, Piper cubeba, Santalum album, and Vitex trifolia) showed a strong activity with 96.4%, 91.9%, 87.6%, 82.6%, and 88.7% inhibition, respectively. Since A. galanga exhibited the most potent activity, its IC50 value was determined with a dose‑dependent effect with the IC50 value of 21.2 µg/mL. Its methanol extract was also separated based on chromatography techniques producing the following four compounds: trans‑p‑coumaryl alcohol (1); p‑coumaryl acetate (2); [1’S]‑1’‑acetoxy chavicol (3); and trans‑p‑coumaryl diacetate (4). Compounds 3 and 4 showed significant activity with the IC50 values of 17.3 and 25.4 µg/mL, respectively. On the other hand, compounds 1 and 2 showed weak activity. All compounds exhibited a similar feature against MCF7, WiDr, and HeLa cell lines. Conclusions: On the structure‑activity relationship observation, acetoxy group at a para position could be a key contributor to the effect. Thus, an acetoxy phenylpropanoid could be a good model for future anti‑breast cancer lead compound development. Furthermore, extract plants should have demonstrated their potential to inhibit cancer cells.
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Da’i, M., Fadhilah, A., Rahmawati, J., Forentin, A. M., Usia, T., & Maryati, M. (2018). T47D Cell‑inhibiting Indonesian Medicinal Plants and Active Constituents of Alpinia galanga Rhizome. Pharmacognosy Magazine, 14(56), 359–363. https://doi.org/10.4103/pm.pm_259_17